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Measurement of O-6-methylguanine-DNA methyltransferase activity using oligonucleotides and restriction enzyme in human brain tumors

  • Authors:
    • T Yanagisawa
    • K Watanabe
    • K Mineura
    • M Kowada

  • View Affiliations

  • Published online on: October 1, 1996     https://doi.org/10.3892/ijo.9.4.781
  • Pages: 781-786
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

We determined the levels of MGMT activity in 46 human brain tumors, using oligonucleotides containing O-6-methylguanine at a PvuII recognition site, and correlated MGMT activity with chemotherapeutic effectiveness in 14 patients with high-grade gliomas who received ACNU chemotherapy. The results characterize MGMT activities among different types and within individual types of human brain tumors. A certain fraction of gliomas exhibited a low value of MGMT activity. Tumors showing a low MGMT activity responded well to ACNU and resulted in prolongation in the time to tumor progression. Thus, the low MGMT activity of gliomas may provide a theoretical basis for application of CENU chemotherapy.

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October 1996
Volume 9 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Journal Metrics
  • Impact Factor: 4.5
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 9.6
  • CiteScore Rank: Q1: #66/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.88
  • SCImago Journal Rank (SJR): 1.099
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Spandidos Publications style
Yanagisawa T, Watanabe K, Mineura K and Kowada M: Measurement of O-6-methylguanine-DNA methyltransferase activity using oligonucleotides and restriction enzyme in human brain tumors. Int J Oncol 9: 781-786, 1996.
APA
Yanagisawa, T., Watanabe, K., Mineura, K., & Kowada, M. (1996). Measurement of O-6-methylguanine-DNA methyltransferase activity using oligonucleotides and restriction enzyme in human brain tumors. International Journal of Oncology, 9, 781-786. https://doi.org/10.3892/ijo.9.4.781
MLA
Yanagisawa, T., Watanabe, K., Mineura, K., Kowada, M."Measurement of O-6-methylguanine-DNA methyltransferase activity using oligonucleotides and restriction enzyme in human brain tumors". International Journal of Oncology 9.4 (1996): 781-786.
Chicago
Yanagisawa, T., Watanabe, K., Mineura, K., Kowada, M."Measurement of O-6-methylguanine-DNA methyltransferase activity using oligonucleotides and restriction enzyme in human brain tumors". International Journal of Oncology 9, no. 4 (1996): 781-786. https://doi.org/10.3892/ijo.9.4.781