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Functional reconstitution of RLIP76 catalyzing ATP-dependent transport of glutathione-conjugates

  • Authors:
    • Sharad S. Singhal
    • Archana Sehrawat
    • Amee Mehta
    • Mukesh Sahu
    • Sanjay Awasthi

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  • Published online on: January 1, 2009     https://doi.org/10.3892/ijo_00000141
  • Pages: 191-199
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Abstract

RLIP76, a stress-responsive, multi-functional protein with multi-specific transport activity towards glutathione-conjugates (GS-E) and chemotherapeutic agents is frequently overexpressed in malignant cells. Our recent studies suggest that it plays a prominent anti-apoptotic role selectively in cancer cells. The present studies were performed to compare RLIP76 activity towards glutathione-conjugates in recombinant and K562 human erythroleukemia cells. The purity and identity of recombinant and K562 RLIP76 was established by SDS-PAGE and Western blot analysis. These studies confirmed the origin of the 38 kDa protein, previously referred to as DNP-SG ATPase, from RLIP76. Comparison of ATPase activity and transport kinetics for DNP-SG and GS-HNE between recombinant vs. K562 RLIP76 revealed higher specific activity of ATPase and transport for recombinant purified RLIP76, indicating that additional factors present in recombinant purified RLIP76 can modulate its transport activity.

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Journal Cover

January 2009
Volume 34 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Impact Factor: 4.5
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  • CiteScore: 9.6
  • CiteScore Rank: Q1: #66/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.88
  • SCImago Journal Rank (SJR): 1.099
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Spandidos Publications style
Singhal SS, Sehrawat A, Mehta A, Sahu M and Awasthi S: Functional reconstitution of RLIP76 catalyzing ATP-dependent transport of glutathione-conjugates. Int J Oncol 34: 191-199, 2009.
APA
Singhal, S.S., Sehrawat, A., Mehta, A., Sahu, M., & Awasthi, S. (2009). Functional reconstitution of RLIP76 catalyzing ATP-dependent transport of glutathione-conjugates. International Journal of Oncology, 34, 191-199. https://doi.org/10.3892/ijo_00000141
MLA
Singhal, S. S., Sehrawat, A., Mehta, A., Sahu, M., Awasthi, S."Functional reconstitution of RLIP76 catalyzing ATP-dependent transport of glutathione-conjugates". International Journal of Oncology 34.1 (2009): 191-199.
Chicago
Singhal, S. S., Sehrawat, A., Mehta, A., Sahu, M., Awasthi, S."Functional reconstitution of RLIP76 catalyzing ATP-dependent transport of glutathione-conjugates". International Journal of Oncology 34, no. 1 (2009): 191-199. https://doi.org/10.3892/ijo_00000141