Localization of cortactin is associated with colorectal cancer development

  • Authors:
    • Hiroshi Hirakawa
    • Kenichiro Shibata
    • Toshiyuki Nakayama
  • View Affiliations

  • Published online on: December 1, 2009     https://doi.org/10.3892/ijo_00000444
  • Pages: 1271-1276
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Abstract

Cortactin is a ubiquitously expressed actin filament (F-actin)-binding protein that stabilizes F-actin networks and promotes actin polymerization by activating the actin-related protein 2/3 (Arp2/3) complex. Overexpression of cortactin in cancer cells stimulate cell migration, invasion, and experimental metastasis; however, the underlying mechanism in cortactin involvement in tumor progression is not fully understood. Recently, a direct interaction between zonula occludens-1 (ZO-1) and cortactin in epithelial cells was reported. The present study aimed to further clarify the significance of the interaction between cortactin and ZO-1 in cancer progression. Cortactin expression and localization in colorectal human cancer tissues were evaluated by immunohistochemistry and immunofluorescence. Co-immunoprecipitation and immunofluorescence analysis revealed cortactin and ZO-1 interaction and localization in cancer cells. In our study, the localization of cortactin is a crucial marker for lymph node metastasis in colorectal cancer. We show how the localization of cortactin effects cancer development. A molecular interaction between cortactin and ZO-1 in migrating or polarized cancer cells was revealed. This is the first report to show the interaction of cortactin and ZO-1 in colorectal cancer progression. We conclude that localization of cortactin in cancer cells and interaction between ZO-1 and cortactin are crucial for cancer progression.

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December 2009
Volume 35 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hirakawa H, Shibata K and Nakayama T: Localization of cortactin is associated with colorectal cancer development. Int J Oncol 35: 1271-1276, 2009.
APA
Hirakawa, H., Shibata, K., & Nakayama, T. (2009). Localization of cortactin is associated with colorectal cancer development. International Journal of Oncology, 35, 1271-1276. https://doi.org/10.3892/ijo_00000444
MLA
Hirakawa, H., Shibata, K., Nakayama, T."Localization of cortactin is associated with colorectal cancer development". International Journal of Oncology 35.6 (2009): 1271-1276.
Chicago
Hirakawa, H., Shibata, K., Nakayama, T."Localization of cortactin is associated with colorectal cancer development". International Journal of Oncology 35, no. 6 (2009): 1271-1276. https://doi.org/10.3892/ijo_00000444