Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism

  • Authors:
    • Mohammad Hashemi
    • Gholamreza Bahari
    • Hedieh Sattarifard
    • Behzad Narouie
  • View Affiliations

  • Published online on: August 8, 2017     https://doi.org/10.3892/mco.2017.1369
  • Pages: 696-700
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Abstract

The present study aimed to examine the impact of a 3-bp indel (rs57408770) polymorphism within the pre‑microRNA (miR)‑3131 polymorphism on prostate cancer (PCa) risk in a sample of an Iranian population. In total, 340 subjects, including 177 patients with PCa and 170 patients with benign prostatic hyperplasia, were enrolled in the present case‑control study. A mismatch polymerase chain reaction‑restriction fragment length polymorphism method was designed for genotyping the 3‑bp indel (rs57408770) polymorphism. The present findings demonstrated that the indel variant significantly increased the risk of PCa in codominant [odds ratio (OR)=2.23, 95% confidence interval (CI)=1.13‑4.37; P=0.021, insertion (ins)/ins vs. deletion (del)/del] and recessive (OR=2.33, 95% CI=1.25‑4.36; P=0.009, ins/ins vs. del/del + del/ins). In conclusion, to the best of our knowledge, the present findings for the first time proposed that a 3‑bp indel variant of miR‑3131 may be a risk factor for susceptibility to PCa in a sample of an Iranian population. Further studies with different ethnicities and larger sample sizes are required to validate the present findings.
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October-2017
Volume 7 Issue 4

Print ISSN: 2049-9450
Online ISSN:2049-9469

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Spandidos Publications style
Hashemi M, Bahari G, Sattarifard H and Narouie B: Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism. Mol Clin Oncol 7: 696-700, 2017
APA
Hashemi, M., Bahari, G., Sattarifard, H., & Narouie, B. (2017). Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism. Molecular and Clinical Oncology, 7, 696-700. https://doi.org/10.3892/mco.2017.1369
MLA
Hashemi, M., Bahari, G., Sattarifard, H., Narouie, B."Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism". Molecular and Clinical Oncology 7.4 (2017): 696-700.
Chicago
Hashemi, M., Bahari, G., Sattarifard, H., Narouie, B."Evaluation of a 3-base pair indel polymorphism within pre-microRNA-3131 in patients with prostate cancer using mismatch polymerase chain reaction-restriction fragment length polymorphism". Molecular and Clinical Oncology 7, no. 4 (2017): 696-700. https://doi.org/10.3892/mco.2017.1369