Open Access

Molecular analysis of circulating tumor DNA from breast cancer patients before and after surgery and following adjuvant chemotherapy

  • Authors:
    • Katarina Zelinova
    • Marianna Jagelkova
    • Zuzana Laucekova
    • Martina Bobrovska
    • Zuzana Dankova
    • Marian Grendar
    • Karol Dokus
  • View Affiliations

  • Published online on: July 20, 2020     https://doi.org/10.3892/mco.2020.2096
  • Article Number: 26
  • Copyright: © Zelinova et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The primary aim of the present study is to provide a complex molecular profile of tumors using liquid biopsy and to monitor profile changes over time in association with surgery and administered adjuvant therapy. Our secondary aim was to compare the liquid biopsy profile with the tissue biopsy and assess concordance. A total of 27 samples of circulating tumor DNA (ctDNA) collected from 9 breast cancer patients at three different time points and their matched formalin‑fixed and paraffin‑embedded (FFPE) samples of primary tumor were analyzed with targeted next‑generation sequencing. Somatic pathogenic variants were detected before surgery in samples from 5 patients (55.6%). The most frequently mutated genes were phosphatase and tensin homolog (4/9, 44.4%) and tumor protein 53 (4/9, 44.4%). Serial sampling of ctDNA enabled the detection of more variants compared with single‑time tissue primary tumor biopsy. There were 17 ctDNA variants across all samples, but only 6 FFPE variants across all patients. In addition, the concordance between ctDNA and FFPE DNA was determined in only 1 patient, and this was connected with higher variant allele frequency. The findings of the present study suggest that liquid biopsy and tissue biopsy may be used as complementary analyses to adequately capture all tumor variants.
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October-2020
Volume 13 Issue 4

Print ISSN: 2049-9450
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Spandidos Publications style
Zelinova K, Jagelkova M, Laucekova Z, Bobrovska M, Dankova Z, Grendar M and Dokus K: Molecular analysis of circulating tumor DNA from breast cancer patients before and after surgery and following adjuvant chemotherapy. Mol Clin Oncol 13: 26, 2020.
APA
Zelinova, K., Jagelkova, M., Laucekova, Z., Bobrovska, M., Dankova, Z., Grendar, M., & Dokus, K. (2020). Molecular analysis of circulating tumor DNA from breast cancer patients before and after surgery and following adjuvant chemotherapy. Molecular and Clinical Oncology, 13, 26. https://doi.org/10.3892/mco.2020.2096
MLA
Zelinova, K., Jagelkova, M., Laucekova, Z., Bobrovska, M., Dankova, Z., Grendar, M., Dokus, K."Molecular analysis of circulating tumor DNA from breast cancer patients before and after surgery and following adjuvant chemotherapy". Molecular and Clinical Oncology 13.4 (2020): 26.
Chicago
Zelinova, K., Jagelkova, M., Laucekova, Z., Bobrovska, M., Dankova, Z., Grendar, M., Dokus, K."Molecular analysis of circulating tumor DNA from breast cancer patients before and after surgery and following adjuvant chemotherapy". Molecular and Clinical Oncology 13, no. 4 (2020): 26. https://doi.org/10.3892/mco.2020.2096