Suppression of A549 lung cancer cell migration by precursor let-7g microRNA

Park,Sungjin; Minai-Tehrani,Arash; Xu,Cheng-Xiong; Chang,Seung-Hee; Woo,Min-Ah; Noh,Mi-Suk; Lee,Eun-Sun; Lim,Hwang-Tae; An,Gil-Hwan; Lee,Kee-Ho; Sung,Ha-Jung; Beck,George R.; Cho,Myung-Haing

doi:10.3892/mmr.2010.373

Suppression of A549 lung cancer cell migration by precursor let-7g microRNA

Authors:
- Sungjin Park
- Arash Minai-Tehrani
- Cheng-Xiong Xu
- Seung-Hee Chang
- Min-Ah Woo
- Mi-Suk Noh
- Eun-Sun Lee
- Hwang-Tae Lim
- Gil-Hwan An
- Kee-Ho Lee
- Ha-Jung Sung
- George R. Beck
- Myung-Haing Cho
View Affiliations

Affiliations: Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea, Department of Nano Fusion Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea, Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, Korea, Laboratory of Molecular Oncology, Korea Institute of Radiological and Medical Sciences, Seoul, Korea, Institute of Fusion Technology, Hoseo University, Chungnam, Korea, Division of Endocrinology, Metabolism and Lipids, Emory University School of Medicine, Atlanta, GA, USA
Published online on: September 27, 2010 https://doi.org/10.3892/mmr.2010.373
Pages: 1007-1013

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Abstract

Let-7g miRNAs, short non-coding RNAs approximately 21 nucleotides long, repress protein translation by binding to the 3'UTR of target mRNAs. Aberrant expression of let-7g is associated with the poor prognosis of lung cancer patients. Compared to normal lung cells, let-7g expression is absent in non-small cell lung cancer (NSCLC) cells. Furthermore, K-Ras and HMGA2 are well known as targets of let-7g. In this study, we evaluated the potential role of precursor (pre)-let-7g in lung cancer cell metastasis, focusing on the two targets of let-7g, HMGA2 and K-Ras. We found that pre-let-7g inhibited the migration of A549 lung cancer cells through HMGA2-mediated E2F1 down-regulation. Thus, our results suggest that pre-let-7g could be used as a suitable target for the suppression of lung cancer cell migration.