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Apoptin sensitizes radiation-induced cell death via classic mitochondrial, caspase and p53-dependent signaling in HepG2 cells

  • Authors:
    • Su-Xia Han
    • Qing Zhu
    • Jin-Lu Ma
    • Yi Lv
    • Jing Zhao
    • Chen Huang
    • Xi Jia
    • Wei Ou
    • Hong-Tao Guo

  • View Affiliations

  • Published online on: October 27, 2010     https://doi.org/10.3892/mmr.2010.391
  • Pages: 59-63
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Resistance or insensitivity to radiation therapy is one of the hallmarks of hepatocellular carcinoma. Sensitizing radioresistant cancer by combining radiation with other therapeutics to induce apoptosis has been widely investigated. Our previous study showed that chicken anaemia virus-derived apoptin protein induced the apoptosis of hepatic carcinoma HepG2 cells. In the present study, we demonstrated that apoptin sensitizes cells to radiation-induced apoptosis using a lentivirus-apoptin expression system in hepatic carcinoma HepG2 cells. Combination therapy with radiation and apoptin dramatically induced mitochondrial cytochrome c release and the cleavage of caspases -9, -3 and -7. Our findings are also the first to show that the combination of radiation and apoptin up-regulates p53 expression. Thus, apoptin treatment represents a potential method for enhancing the effectiveness of radiotherapy in poorly responding hepatocellular carcinoma.

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January-February 2011
Volume 4 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 6.9
  • CiteScore Rank: Q2: #98/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.721
  • SCImago Journal Rank (SJR): 0.65
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Spandidos Publications style
Han S, Zhu Q, Ma J, Lv Y, Zhao J, Huang C, Jia X, Ou W and Guo H: Apoptin sensitizes radiation-induced cell death via classic mitochondrial, caspase and p53-dependent signaling in HepG2 cells. Mol Med Rep 4: 59-63, 2011
APA
Han, S., Zhu, Q., Ma, J., Lv, Y., Zhao, J., Huang, C. ... Guo, H. (2011). Apoptin sensitizes radiation-induced cell death via classic mitochondrial, caspase and p53-dependent signaling in HepG2 cells. Molecular Medicine Reports, 4, 59-63. https://doi.org/10.3892/mmr.2010.391
MLA
Han, S., Zhu, Q., Ma, J., Lv, Y., Zhao, J., Huang, C., Jia, X., Ou, W., Guo, H."Apoptin sensitizes radiation-induced cell death via classic mitochondrial, caspase and p53-dependent signaling in HepG2 cells". Molecular Medicine Reports 4.1 (2011): 59-63.
Chicago
Han, S., Zhu, Q., Ma, J., Lv, Y., Zhao, J., Huang, C., Jia, X., Ou, W., Guo, H."Apoptin sensitizes radiation-induced cell death via classic mitochondrial, caspase and p53-dependent signaling in HepG2 cells". Molecular Medicine Reports 4, no. 1 (2011): 59-63. https://doi.org/10.3892/mmr.2010.391