hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas

Wang,Yi; Luo,Hongmei; Li,Yangle; Chen,Tieding; Wu,Shengpeng; Yang,Luoyan

doi:10.3892/mmr.2011.621

hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas

Authors:
- Yi Wang
- Hongmei Luo
- Yangle Li
- Tieding Chen
- Shengpeng Wu
- Luoyan Yang
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Affiliations: Department of Urology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R China, Department of Histology and Embryology, University of South China, Hengyang, Hunan 421001, P.R. China
Published online on: October 11, 2011 https://doi.org/10.3892/mmr.2011.621
Pages: 260-265

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Abstract

Numerous microRNAs (miRNAs) play crucial roles in cancer development. In this study, we report that hsa-miR-96 is expressed at higher levels in human bladder urothelial carcinomas compared to normal tissues. We found that hsa-miR-96 increased invasion and differentiation of human bladder T24 cells and promoted their growth. Down‑regulation of hsa-miR-96 significantly affected the phenotype of bladder cancer T24 cells. The mRNA and protein levels of insulin receptor substrate 1 (IRS1) and MAP4K1 were significantly reduced in cells transfected with the hsa-miR-96 inhibitor when compared with levels in cells transfected with the empty plasmid vector or the negative control miRNA inhibitor. Altogether, these results suggest that hsa-miR-96 may affect the growth of bladder cancer cells by up-regulating IRS1 and MAP4K1 levels, functioning as a promising diagnostic marker in human bladder urothelial carcinomas.