Th1/Th2/Th17/Treg expression in cultured PBMCs with antiphospholipid antibodies

  • Authors:
    • Jing Xiao
    • Fufan Zhu
    • Xinli Liu
    • Jing Xiong
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  • Published online on: August 30, 2012     https://doi.org/10.3892/mmr.2012.1055
  • Pages: 1035-1039
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Abstract

The aim of this study was to evaluate the expression of T-helper cell subtypes Th1, Th2, Th17 and Treg in antiphospholipid syndrome (APS), and whether they are related to anti-cardiolipin antibody (aPL) titers. Peripheral mononuclear cells (PBMCs) were isolated from healthy donors, and incubated with aPLs. Subsequent to a 48‑h incubation, PBMCs were collected and detected by flow cytometry. The results revealed that aPLs at higher concentrations may induce a significant increase in Th2 and Th17 frequencies, as opposed to a significant decrease in Th1 and Treg frequencies and the Th1/Th2 ratio. These results indicate that there is a Th1/Th2 imbalance, a Th17 upregulation and a Treg downregulation present in APS, and that these factors are positively correlated with aPL titers, suggesting a potential role of Th cells in the pathogenesis of APS.
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November 2012
Volume 6 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xiao J, Zhu F, Liu X and Xiong J: Th1/Th2/Th17/Treg expression in cultured PBMCs with antiphospholipid antibodies. Mol Med Rep 6: 1035-1039, 2012
APA
Xiao, J., Zhu, F., Liu, X., & Xiong, J. (2012). Th1/Th2/Th17/Treg expression in cultured PBMCs with antiphospholipid antibodies. Molecular Medicine Reports, 6, 1035-1039. https://doi.org/10.3892/mmr.2012.1055
MLA
Xiao, J., Zhu, F., Liu, X., Xiong, J."Th1/Th2/Th17/Treg expression in cultured PBMCs with antiphospholipid antibodies". Molecular Medicine Reports 6.5 (2012): 1035-1039.
Chicago
Xiao, J., Zhu, F., Liu, X., Xiong, J."Th1/Th2/Th17/Treg expression in cultured PBMCs with antiphospholipid antibodies". Molecular Medicine Reports 6, no. 5 (2012): 1035-1039. https://doi.org/10.3892/mmr.2012.1055