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December 2012 Volume 6 Issue 6

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Article

Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro

  • Authors:
    • Sheyu Lin
    • Junyu Zhang
    • Jiawei Xu
    • Honglian Wang
    • Qing Sang
    • Qinghe Xing
    • Lin He
  • View Affiliations / Copyright

    Affiliations: School of Life Sciences, Nantong University, Nantong 226019, P.R. China, Children's Hospital, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, P.R. China
  • Pages: 1243-1248
    |
    Published online on: September 26, 2012
       https://doi.org/10.3892/mmr.2012.1099
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Abstract

The SAM and SH3 domain containing 1 (SASH1) gene was originally identified as a potential tumor suppressor gene in breast cancer, mapped on chromosome 6q24.3. The expression of SASH1 plays a prognostic role in human colon cancer. Its expression is frequently downregulated in several human malignancies. However, the biological function of SASH1 in melanoma cells is yet to be determined. In this study, in order to investigate the tumor suppressive effects of the SASH1 gene, an A-375 stable melanoma cell line was established, overexpressing the SASH1 gene. The stable cell line was examined using proliferation assay, apoptosis assay, cell cycle analysis and real-time PCR. The results indicated that the tumor suppressive activity of SASH1 derived from G2/M arrest in A-375 cells, and that the phosphorylation of Cdc2 or the disruption of cyclin B-Cdc2 binding may be responsible for the G2/M arrest.
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Copy and paste a formatted citation
Spandidos Publications style
Lin S, Zhang J, Xu J, Wang H, Sang Q, Xing Q and He L: Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro. Mol Med Rep 6: 1243-1248, 2012.
APA
Lin, S., Zhang, J., Xu, J., Wang, H., Sang, Q., Xing, Q., & He, L. (2012). Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro. Molecular Medicine Reports, 6, 1243-1248. https://doi.org/10.3892/mmr.2012.1099
MLA
Lin, S., Zhang, J., Xu, J., Wang, H., Sang, Q., Xing, Q., He, L."Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro". Molecular Medicine Reports 6.6 (2012): 1243-1248.
Chicago
Lin, S., Zhang, J., Xu, J., Wang, H., Sang, Q., Xing, Q., He, L."Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro". Molecular Medicine Reports 6, no. 6 (2012): 1243-1248. https://doi.org/10.3892/mmr.2012.1099
Copy and paste a formatted citation
x
Spandidos Publications style
Lin S, Zhang J, Xu J, Wang H, Sang Q, Xing Q and He L: Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro. Mol Med Rep 6: 1243-1248, 2012.
APA
Lin, S., Zhang, J., Xu, J., Wang, H., Sang, Q., Xing, Q., & He, L. (2012). Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro. Molecular Medicine Reports, 6, 1243-1248. https://doi.org/10.3892/mmr.2012.1099
MLA
Lin, S., Zhang, J., Xu, J., Wang, H., Sang, Q., Xing, Q., He, L."Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro". Molecular Medicine Reports 6.6 (2012): 1243-1248.
Chicago
Lin, S., Zhang, J., Xu, J., Wang, H., Sang, Q., Xing, Q., He, L."Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro". Molecular Medicine Reports 6, no. 6 (2012): 1243-1248. https://doi.org/10.3892/mmr.2012.1099
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