Effect of radiation on the expression of osteoclast marker genes in RAW264.7 cells

Yang,Bing; Zhou,Hui; Zhang,Xiao-Dong; Liu,Zheng; Fan,Fei-Yue; Sun,Yuan-Ming

doi:10.3892/mmr.2012.765

Effect of radiation on the expression of osteoclast marker genes in RAW264.7 cells

Authors:
- Bing Yang
- Hui Zhou
- Xiao-Dong Zhang
- Zheng Liu
- Fei-Yue Fan
- Yuan-Ming Sun
View Affiliations

Affiliations: Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, P.R. China, School of Pharmacy, Tianjin Medical University, Tianjin 300070, P.R. China
Published online on: January 25, 2012 https://doi.org/10.3892/mmr.2012.765
Pages: 955-958

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Cancer radiation therapy can cause skeletal complications, such as osteopenia and osteoporosis. To understand the mechanism responsible for the skeletal complications, the expression profiles of osteoclast marker genes in RAW264.7 cells were observed. Osteoclast formation was established by RAW264.7 cells that were treated with the receptor activator of nuclear factor (NF)-κB ligand (RANKL) and detected using immunochemistry and morphological observations. Quantitative real-time polymerase chain reaction was used to assess the expression of a panel of osteoclast markers, including the receptor activator of NF-κB (RANK), tartrate-resistant acid phosphatase (TRAP), integrin β3 and the calcitonin receptor (CTR). RANKL-induced osteoclasts were TRAP-positive and multinucleated, and displayed a distinct morphology. RANKL-induced osteoclast precursor cells had increased TRAP and RANK expression and decreased CTR expression compared to the control cells not treated with RANKL. RAW264.7 cells irradiated with 2-Gy γ-rays had upregulated integrin β3 and RANK expression and downregulated CTR expression compared to the control RAW264.7 cells. The effect of radiation on RANKL-induced osteoclast differentiation enhanced the expression of CTR and inhibited the expression of RANK and TRAP. Therefore, radiation damage from 2-Gy γ-rays can promote the activities of osteoclast precursor cells, but not those of osteoclasts.

View Figures

View References

1	A BanfiG BianchiM GalottoR CanceddaR QuartoBone marrow stromal damage after chemo/radiotherapy: occurrence, consequences and possibilities of treatmentLeuk Lymphoma42863870200110.3109/1042819010909770511697641
2	NN BaxterEB HabermannJE TepperSB DurhamBA VirnigRisk of pelvic fractures in older women following pelvic irradiationJAMA29425872593200510.1001/jama.294.20.258716304072
3	KH DarzySM ShaletHypopituitarism after cranial irradiationJ Endocrinol Invest287887200516114281
4	AO LanglandsWA SouterE SamuelAT RedpathRadiation osteitis following irradiation for breast cancerClin Radiol289396197710.1016/S0009-9260(77)80134-7852230
5	BL CuetaraTN CrottiAJ O'DonoghueKP McHughCloning and characterization of osteoclast precursors from the RAW264.7 cellineIn Vitro Cell Dev Biol Anim42182188200610.1290/0510075.1
6	M ItoN MatsukaM IzukaCharacterization of inorganic phosphate transport in osteoclast-like cellsAm J Physiol Cell Physiol288C921C931200510.1152/ajpcell.00412.200415601753
7	H HsuDL LaceyCR DunstanTumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligandProc Natl Acad Sci USA9635403545199910.1073/pnas.96.7.3540
8	TL BurgessY QianS KaufmanThe ligand for osteoprotegerin (OPGL) directly activates mature osteoclastsJ Cell Biol145527538199910.1083/jcb.145.3.52710225954
9	S MakihiraY MineE KosakaH NikawaTitanium surface roughness accelerates RANKL-dependent differentiation in the osteoclast precursor cell line, RAW264.7Dent Mater J26739745200710.4012/dmj.26.73918203477
10	DR HaynesTN CrottiH ZreiqatRegulation of osteoclast activity in peri-implant tissuesBiomaterials2548774885200410.1016/j.biomaterials.2004.01.00315109848
11	M AsagiriH TakayanagiThe molecular understanding of osteoclast differentiationBone40251264200710.1016/j.bone.2006.09.02317098490
12	JS WilleyEW LivingstonME RobbinsJD BourlandL Tirado-LeeH Smith-SielickiTA BatemanRisedronate prevents early radiation-induced osteoporosis in mice at multiple skeletal locationsBone46101111201010.1016/j.bone.2009.09.00219747571
13	K HollbergJ NordahlK HultenbyS Mengarelli-WidholmG AnderssonFP ReinholtPolarization and secretion of cathepsin K precede tartrate-resistant acid phosphatase secretion to the ruffled border area during the activation of matrix-resorbing clastsJ Bone Miner Metab23441449200510.1007/s00774-005-0626-316261450
14	B KirsteinTJ ChambersK FullerSecretion of tartrate-resistant acid phosphatase by osteoclasts correlates with resorptive behaviorJ Cell Biochem9810851094200610.1002/jcb.2083516475168
15	G AnderssonB Ek-RylanderK HollbergTRACP as an osteopontin phosphataseJ Bone Miner Res1819121915200310.1359/jbmr.2003.18.10.191214584906
16	A SuterV EvertsA BoydeOverlapping functions of lysosomal acid phosphatase (LAP) and tartrate-resistant acid phosphatase (Acp5) revealed by doubly deficient miceDevelopment12848994910200111731469
17	NC WalshM CahillP CarninciMultiple tissue-specific promoters control expression of the murine tartrate-resistant acid phosphatase geneGene307111123200310.1016/S0378-1119(03)00449-912706893
18	R BattaglinoD KimJ FuB VaageXY FuP Stashenkoc-myc is required for osteoclast differentiationJ Bone Miner Res17763773200210.1359/jbmr.2002.17.5.76312009006
19	SL TeitelbaumFP RossGenetic regulation of osteoclast development and functionNat Rev Genet4638649200310.1038/nrg112212897775
20	FP RossJ ChappelJI AlvarezInteractions between the bone matrix proteins osteopontin and bone sialoprotein and the osteoclast integrin alpha v beta 3 potentiate bone resorptionJ Biol Chem2689901990719938486670
21	D HuC LuA SapozhnikovaM BarnettC SparreyT MiclauRS MarcucioThe absence of beta-3 integrin accelerates early skeletal repairJ Orthop Res283237201019637214
22	GC NicholsonJM MoseleyPM SextonFA MendelsohnTJ MartinAbundant calcitonin receptors in isolated rat osteoclasts. Biochemical and autoradiographic characterizationJ Clin Invest78355360198610.1172/JCI1125843016026
23	M ZaidiM PazianasVS ShankarOsteoclast function and its controlExp Physiol78721739199310.1113/expphysiol.1993.sp0037218311941
24	JM QuinnM MorfisMH LamCalcitonin receptor antibodies in the identification of osteoclastsBone2518199910.1016/S8756-3282(99)00094-010423015
25	J CornishKE CallonU BavaSA KamonaGJ CooperIR ReidEffects of calcitonin, amylin, and calcitonin gene-related peptide on osteoclast developmentBone29162168200110.1016/S8756-3282(01)00494-X11502478
26	SK LeeSR GoldringJA LorenzoExpression of the calcitonin receptor in bone marrow cell cultures and in bone: a specific marker of the differentiated osteoclast that is regulated by calcitoninEndocrinology1364572458119957664679
27	WJ BoyleWS SimonetDL LaceyOsteoclast differentiation and activationNature423337342200310.1038/nature0165812748652
28	J LiI SarosiXQ YanRANK is the intrinsic hematopoietic cell surface receptor that controls osteoclastogenesis and regulation of bone mass and calcium metabolismProc Natl Acad Sci USA9715661571200010.1073/pnas.97.4.156610677500
29	WC DougallM GlaccumK CharrierRANK is essential for osteoclast and lymph node developmentGenes Dev1324122424199910.1101/gad.13.18.241210500098