Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome

  • Authors:
    • Jin-Jian Lu
    • Zhen Yang
    • De-Zhao Lu
    • Xing-De Wo
    • Jia‑Jie  Shi
    • Tao‑Qi Lin
    • Mi-Mi Wang
    • Yi Li
    • Li-Hua Tang
  • View Affiliations

  • Published online on: May 8, 2012     https://doi.org/10.3892/mmr.2012.906
  • Pages: 429-433
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Abstract

Artemisinin, the active ingredient of the Chinese medicinal herb Artemisia annua L., and its derivatives (ARTs) are currently widely used as anti-malarial drugs around the world. In this study, we found that dihydroartemisinin (DHA), one of the main active metabolites of ARTs, inhibited the proliferation of human hepatocarcinoma BEL-7402 cells in a concentration-dependent manner. To interpret the mechanisms involved, an analysis of the mitochondrial proteome was performed employing two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Seven mitochondrial proteins including fumarate hydratase, 60 kDa heat shock protein, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, two subunits of ATP synthase and NADPH:adrenodoxin oxidoreductase were identified to be differentially expressed between the control and DHA-treated groups. Our results indicate that the imbalance of energy metabolism induced by DHA may contribute, at least in part, to its anti-cancer potential in BEL-7402 cells.
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August 2012
Volume 6 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lu J, Yang Z, Lu D, Wo X, Shi JJ, Lin TQ, Wang M, Li Y and Tang L: Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome. Mol Med Rep 6: 429-433, 2012.
APA
Lu, J., Yang, Z., Lu, D., Wo, X., Shi, J., Lin, T. ... Tang, L. (2012). Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome. Molecular Medicine Reports, 6, 429-433. https://doi.org/10.3892/mmr.2012.906
MLA
Lu, J., Yang, Z., Lu, D., Wo, X., Shi, J., Lin, T., Wang, M., Li, Y., Tang, L."Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome". Molecular Medicine Reports 6.2 (2012): 429-433.
Chicago
Lu, J., Yang, Z., Lu, D., Wo, X., Shi, J., Lin, T., Wang, M., Li, Y., Tang, L."Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome". Molecular Medicine Reports 6, no. 2 (2012): 429-433. https://doi.org/10.3892/mmr.2012.906