Effects of cyclin E gene silencing on the proliferation of esophageal cancer cell lines, EC9706, Eca109 and KYSE30 Retraction in /10.3892/mmr.2018.8406

Wang,Na; Li,Min; Zang,Wen-Qiao; Ma,Yun-Yun; Wang,Yuan-Yuan; Zhao,Guo-Qiang

doi:10.3892/mmr.2013.1280

Effects of cyclin E gene silencing on the proliferation of esophageal cancer cell lines, EC9706, Eca109 and KYSE30

Retraction in: /10.3892/mmr.2018.8406

Authors:
- Na Wang
- Min Li
- Wen-Qiao Zang
- Yun-Yun Ma
- Yuan-Yuan Wang
- Guo-Qiang Zhao
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Affiliations: Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, P.R. China, Department of Immunology and Microbiology, Henan Medical College for Staff and Workers, Zhengzhou 451191, P.R. China
Published online on: January 18, 2013 https://doi.org/10.3892/mmr.2013.1280
Pages: 799-804

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Abstract

In order to observe the effects of cyclin E gene silencing by small interfering RNA (siRNA) on the growth, proliferation, invasion and apoptosis of esophageal cancer cell lines, including EC9706, Eca109 and KYSE30, siRNA vectors targeting cyclin E gene were constructed and then transfected into the EC9706, Eca109 and KYSE30 human esophageal cancer cell lines. Cyclin E mRNA and protein expression were determined by RT-PCR and western blotting. Cell proliferation and clonality were detected using a CCK-8 test and soft agar colony formation assay. Cell cycle distribution, apoptosis and invasion of EC9706, Eca109 and KYSE30 cells were evaluated with flow cytometry and a transwell culture system. After siRNA vectors targeting the cyclin E gene were transfected into EC9706, Eca109 and KYSE30 cell lines, compared with blank and negative control groups, the expression of cyclin E mRNA and protein (P<0.01), colony-forming units and the number of cells penetrating the transwell membrane (P<0.05) were significantly decreased, the cells in the S and G2/M phase were reduced, the cells in the G0/G1 phase were increased and the apoptosis rate was increased (P<0.01) in the experimental groups. Cyclin E gene silencing effectively inhibits growth, proliferation and invasion of esophageal cancer cells.

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