Recombinant Ganoderma lucidum immunomodulatory protein modified with polyethylene glycol

  • Authors:
    • Xiping Zhang
    • Fei Sun
    • Zhiyi Liu
    • Shuqin Zhang
    • Chongyang Liang
  • View Affiliations

  • Published online on: January 18, 2013     https://doi.org/10.3892/mmr.2013.1281
  • Pages: 975-980
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Abstract

Recombinant Ganoderma lucidum immunomodu­latory protein (rLZ-8) expressed using the Pichia yeast eukaryotic expression system is a potential new drug for cancer therapy; however, it has a short half-life in the body. In order to optimize the potency and stability of rLZ-8, we modified the recombinant protein chemically using methoxy-PEG-succinimidyl propionate (mPEG-SPA). The results indicated that several parameters, including pH, the molar ratio of rLZ-8 to mPEG-SPA and time, played crucial roles in the modification process. In particular, when the molar ratio of rLZ-8 to mPEG-SPA was 1:1, rLZ-8 was modified by a single mPEG-SPA moiety. In addition, MALDI-TOF/TOF and ESI Q-Trap results revealed that the difference in molecular weight (MW) between the peptide-linked mPEG-SPA and the mPEG-SPA closely matched the MW of a methionine amino acid. Taken together, these data suggest that modification of mPEG-SPA occurred on the N-terminal helix of rLZ-8. This modification method has laid a foundation for the development of long-acting formulations of rLZ-8.
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March 2013
Volume 7 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang X, Sun F, Liu Z, Zhang S and Liang C: Recombinant Ganoderma lucidum immunomodulatory protein modified with polyethylene glycol. Mol Med Rep 7: 975-980, 2013.
APA
Zhang, X., Sun, F., Liu, Z., Zhang, S., & Liang, C. (2013). Recombinant Ganoderma lucidum immunomodulatory protein modified with polyethylene glycol. Molecular Medicine Reports, 7, 975-980. https://doi.org/10.3892/mmr.2013.1281
MLA
Zhang, X., Sun, F., Liu, Z., Zhang, S., Liang, C."Recombinant Ganoderma lucidum immunomodulatory protein modified with polyethylene glycol". Molecular Medicine Reports 7.3 (2013): 975-980.
Chicago
Zhang, X., Sun, F., Liu, Z., Zhang, S., Liang, C."Recombinant Ganoderma lucidum immunomodulatory protein modified with polyethylene glycol". Molecular Medicine Reports 7, no. 3 (2013): 975-980. https://doi.org/10.3892/mmr.2013.1281