The effect of a proton pump inhibitor on bone metabolism in ovariectomized rats

  • Authors:
    • Moon Kyung Joo
    • Jong-Jae Park
    • Beom Jae Lee
    • Ji Hoon Kim
    • Jong Eun Yeon
    • Jae Seon Kim
    • Kwan Soo Byun
    • Young-Tae Bak
  • View Affiliations

  • Published online on: February 19, 2013     https://doi.org/10.3892/mmr.2013.1327
  • Pages: 1267-1272
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Abstract

Recent studies revealed that long-term intake of proton pump inhibitor (PPI) increases the risk of vertebral or hip fracture; however, the exact mechanism for this is not known. To evaluate the effect of long-term PPI therapy on bone turnover, we analyzed the signaling pathway involved in osteoclast differentiation and bone resorption/formation markers using ovariectomized rats. Six-week-old Sprague-Dawley (S-D) rats were ovariectomized, and two weeks later they were divided into four groups (group A, normal diet + placebo; group B, low calcium diet + placebo; group C, normal diet + PPI; and group D, low calcium diet + PPI). Omeprazole, at a concentration of 30 mg/kg, was administered orally for eight weeks and the rats were sacrificed when they were 16 weeks old. The relative expression levels of the receptor activator of NF-κB ligand (RANKL)/osteoprotegerin (OPG) ratio, c-Fos, nuclear factor of activated T cells c1 (NFATc1) and osteocalcin in femoral bone marrow cells were compared, and serum C-terminal cross­linking telopeptide of type I (CTX-1) levels were determined. The relative ratio of RANKL/OPG was increased in group D, and gene expression levels of c-Fos and NFATc1 were upregulated in groups B and D, which are involved in differentiation and activation of osteoclasts. Furthermore, expression levels of osteocalcin, a bone formation marker, were decreased and levels of serum CTX-1, a bone resorption marker, were increased in group D. Taken together, a low calcium diet and PPI administration are thought to collaborate in order to alter osteoclast activity and bone resorption signaling.
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April 2013
Volume 7 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Joo MK, Park J, Lee BJ, Kim JH, Yeon JE, Kim JS, Byun KS and Bak Y: The effect of a proton pump inhibitor on bone metabolism in ovariectomized rats. Mol Med Rep 7: 1267-1272, 2013.
APA
Joo, M.K., Park, J., Lee, B.J., Kim, J.H., Yeon, J.E., Kim, J.S. ... Bak, Y. (2013). The effect of a proton pump inhibitor on bone metabolism in ovariectomized rats. Molecular Medicine Reports, 7, 1267-1272. https://doi.org/10.3892/mmr.2013.1327
MLA
Joo, M. K., Park, J., Lee, B. J., Kim, J. H., Yeon, J. E., Kim, J. S., Byun, K. S., Bak, Y."The effect of a proton pump inhibitor on bone metabolism in ovariectomized rats". Molecular Medicine Reports 7.4 (2013): 1267-1272.
Chicago
Joo, M. K., Park, J., Lee, B. J., Kim, J. H., Yeon, J. E., Kim, J. S., Byun, K. S., Bak, Y."The effect of a proton pump inhibitor on bone metabolism in ovariectomized rats". Molecular Medicine Reports 7, no. 4 (2013): 1267-1272. https://doi.org/10.3892/mmr.2013.1327