Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3

  • Authors:
    • Fengsheng Li
    • Ling Gao
    • Qisheng Jiang
    • Zhidong Wang
    • Bo Dong
    • Tao Yan
    • Xiaohua Chen
  • View Affiliations

  • Published online on: April 25, 2013     https://doi.org/10.3892/mmr.2013.1441
  • Pages: 1883-1888
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In the present study, the effect of radiation on the invasion of the pulmonary adenocarcinoma cell line, A549, was investigated. Invasion of A549 cells irradiated with 2 and 4 Gy doses of γ‑ray was detected using the transwell Matrigel invasion assay. Levels of matrix metalloproteinase 2 (MMP‑2) and phosphorylated signal transducer and activator of transcription 3 (STAT3) were detected by reverse transcription PCR (RT-PCR) and/or immunoblotting. The enzyme activity of MMP‑2 was examined by gelatin zymography. Results demonstrated that the invasion of A549 cells was significantly enhanced by γ‑ray radiation at doses of 2 or 4 Gy. In addition, exposure to radiation was found to promote transcriptional expression of MMP‑2 and increase MMP‑2 enzyme activity. Irradiation activated the phosphorylation of STAT3 and promoted the nuclear localization of STAT3. The blockage of STAT3 phosphorylation using a specific inhibitor (AG490) suppressed the irradiation‑induced elevation of MMP‑2 expression, enzyme activity and invasion of A549 cells. Finally, the expression of vascular endothelial growth factor (VEGF) was found to be upregulated by radiation, which was associated with the activation of STAT3. Results of the current study indicate that irradiation leads to activation of STAT3 and translocation to the nucleus, leading to activation of VEGF and MMP‑2 transcription, resulting in the increased invasion of A549 cells.
View Figures
View References

Related Articles

Journal Cover

June 2013
Volume 7 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li F, Gao L, Jiang Q, Wang Z, Dong B, Yan T and Chen X: Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3. Mol Med Rep 7: 1883-1888, 2013.
APA
Li, F., Gao, L., Jiang, Q., Wang, Z., Dong, B., Yan, T., & Chen, X. (2013). Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3. Molecular Medicine Reports, 7, 1883-1888. https://doi.org/10.3892/mmr.2013.1441
MLA
Li, F., Gao, L., Jiang, Q., Wang, Z., Dong, B., Yan, T., Chen, X."Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3". Molecular Medicine Reports 7.6 (2013): 1883-1888.
Chicago
Li, F., Gao, L., Jiang, Q., Wang, Z., Dong, B., Yan, T., Chen, X."Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3". Molecular Medicine Reports 7, no. 6 (2013): 1883-1888. https://doi.org/10.3892/mmr.2013.1441