Anti-inflammatory effects of essential oils from Chamaecyparis obtusa via the cyclooxygenase-2 pathway in rats

An,Beum-Soo; Kang,Ji-Houn; Yang,Hyun; Jung,Eui-Man; Kang,Hong-Seok; Choi,In-Gyu; Park,Mi-Jin; Jeung,Eui-Bae

doi:10.3892/mmr.2013.1459

Anti-inflammatory effects of essential oils from Chamaecyparis obtusa via the cyclooxygenase-2 pathway in rats

Authors:
- Beum-Soo An
- Ji-Houn Kang
- Hyun Yang
- Eui-Man Jung
- Hong-Seok Kang
- In-Gyu Choi
- Mi-Jin Park
- Eui-Bae Jeung
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Affiliations: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea, Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea , Department of Biomaterials Science, College of National Resources and Life Science, Pusan National University, Miryang, Gyeongsangnam-do 627-706, Republic of Korea, Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea, Department of Forest Products, Division of Wood Chemistry and Microbiology, Korea Forest Research Institute, Seoul 130-712, Republic of Korea
Published online on: May 8, 2013 https://doi.org/10.3892/mmr.2013.1459
Pages: 255-259

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Abstract

Essential oils are concentrated hydrophobic liquids containing volatile aromatic compounds from plants. In the present study, the essential oil of Chamaecyparis obtusa (C. obtusa), which is commercially used in soap, toothpaste and cosmetics, was extracted. Essential oil extracted from C. obtusa contains several types of terpenes, which have been shown to have anti-oxidative and anti-inflammatory effects. In the present study, we examined the anti-inflammatory effects of C. obtusa essential oil in vivo and in vitro following the induction of inflammation by lipopolysaccharides (LPS) in rats. While LPS induced an inflammatory response through the production of prostaglandin E2 (PGE2) in the blood and peripheral blood mononuclear cells (PMNCs), these levels were reduced when essential oil was pre-administered. Additionally, the mechanism of action underlying the anti-inflammatory effects of C. obtusa essential oil was investigated by measuring the mRNA expression of inflammation‑associated genes. LPS treatment significantly induced the expression of transforming growth factor α (TNFα) and cyclooxygenase-2 (COX-2) in rats, while C. obtusa essential oil inhibited this effect. Taken together, our results demonstrate that C. obtusa essential oil exerts anti‑inflammatory effects by regulating the production of PGE2 and TNFα gene expression through the COX-2 pathway. These findings suggest that C. obtusa essential oil may constitute a novel source of anti-inflammatory drugs.

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