Lefty A protein inhibits TGF-β1-mediated apoptosis in human renal tubular epithelial cells

  • Authors:
    • Ren-Ping Zheng
    • Tao Bai
    • Xiao-Guang Zhou
    • Chang-Gen Xu
    • Wei Wang
    • Ming-Wei Xu
    • Jie Zhang
  • View Affiliations

  • Published online on: June 26, 2013     https://doi.org/10.3892/mmr.2013.1556
  • Pages: 621-625
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Abstract

This study aimed to examine the effects of Lefty A protein on transforming growth factor-β1 (TGF-β1)-mediated apoptosis in human renal tubular epithelial cells (HK-2). HK-2 cells were transfected with the human Lefty gene to induce the secretion of endogenous Lefty A protein. Following exposure of the HK-2 cells to recombinant human TGF-β1 (10 ng/ml), p-Smad2/3 protein levels were examined by western blot analysis, and cellular apoptosis was detected by flow cytometry 6, 12, 24 and 48 h following TGF-β1 treatment. Coculture of renal tubular epithelial cells with TGF-β1 resulted in a significant increase in p-Smad2/3 protein levels and the rate of cell apoptosis, which were attenuated by liposome-mediated transfection with the Lefty gene. Lefty A protein was able to inhibit the TGF-β1/Smad signaling pathway and markedly attenuate TGF-β1-mediated apoptosis in human renal tubular epithelial cells. Taken together, these results indicated that the TGF-β1/Smad signaling pathway most likely mediates apoptosis in renal tubular epithelial cells. In addition, Lefty A protein is capable of inhibiting the TGF-β1/Smad pathway to reduce TGF-β1/Smad-mediated apoptosis in renal tubular epithelial cells. This study may provide novel insights into the prevention and treatment of urinary tract obstruction disease using Lefty A protein.
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August 2013
Volume 8 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zheng R, Bai T, Zhou X, Xu C, Wang W, Xu M and Zhang J: Lefty A protein inhibits TGF-β1-mediated apoptosis in human renal tubular epithelial cells. Mol Med Rep 8: 621-625, 2013.
APA
Zheng, R., Bai, T., Zhou, X., Xu, C., Wang, W., Xu, M., & Zhang, J. (2013). Lefty A protein inhibits TGF-β1-mediated apoptosis in human renal tubular epithelial cells. Molecular Medicine Reports, 8, 621-625. https://doi.org/10.3892/mmr.2013.1556
MLA
Zheng, R., Bai, T., Zhou, X., Xu, C., Wang, W., Xu, M., Zhang, J."Lefty A protein inhibits TGF-β1-mediated apoptosis in human renal tubular epithelial cells". Molecular Medicine Reports 8.2 (2013): 621-625.
Chicago
Zheng, R., Bai, T., Zhou, X., Xu, C., Wang, W., Xu, M., Zhang, J."Lefty A protein inhibits TGF-β1-mediated apoptosis in human renal tubular epithelial cells". Molecular Medicine Reports 8, no. 2 (2013): 621-625. https://doi.org/10.3892/mmr.2013.1556