Lack of effect of common single nucleotide polymorphisms in leukotriene pathway genes on platelet reactivity in patients with diabetes

Rosiak,Marek; Postula,Marek; Kaplon‑Cieslicka,Agnieszka; Kondracka,Agnieszka; Trzepla,Ewa; Zaremba,Malgorzata; Filipiak,Krzysztof  J.; Kosior,Dariusz  A.; Czlonkowski,Andrzej; Opolski,Grzegorz; Janicki,Piotr K.

doi:10.3892/mmr.2013.1567

Lack of effect of common single nucleotide polymorphisms in leukotriene pathway genes on platelet reactivity in patients with diabetes

Authors:
- Marek Rosiak
- Marek Postula
- Agnieszka Kaplon‑Cieslicka
- Agnieszka Kondracka
- Ewa Trzepla
- Malgorzata Zaremba
- Krzysztof J. Filipiak
- Dariusz A. Kosior
- Andrzej Czlonkowski
- Grzegorz Opolski
- Piotr K. Janicki
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Affiliations: Department of Cardiology, Medical University of Warsaw, Warsaw 02‑927, Poland, Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw 02‑927, Poland, Department of Internal Medicine and Endocrinology, Medical University of Warsaw, Warsaw 02‑927, Poland, Department of Noninvasive Cardiology and Hypertension, Central Clinical Hospital, The Ministry of the Interior, Warsaw 02‑507, Poland, Perioperative Genomics Laboratory, Department of Anesthesiology, Penn State College of Medicine, Hershey, Pennsylvania 17033‑0850, USA
Published online on: July 3, 2013 https://doi.org/10.3892/mmr.2013.1567
Pages: 853-860

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Abstract

The aim of the present study was to investigate the effect of genetic polymorphisms in candidate genes within the leukotriene (LT) pathway on platelet reactivity and the concentration of selected LTs in diabetic patients treated with acetylsalicylic acid (ASA). The study cohort consisted of 287 Caucasians with type 2 diabetes who had received treatment with ASA tablets (75 mg/day) for at least three months. Platelet reactivity analyses were performed using VerifyNow aspirin and PFA‑100 assays. The measured LTs included leukotriene B4 (LTB4) and leukotriene E4 (LTE4). Genotyping for the selected 25 single nucleotide polymorphisms (SNPs) within six genes of the LT pathway was performed using a Sequenom iPLEX platform. No statistically significant association was observed between the investigated SNP genotypes, platelet reactivity and measured LTs in the patient cohort. The results of our study suggest that certain polymorphisms of the LT pathway are not associated with altered platelet reactivity and the measured LTs in diabetic patients treated with ASA.

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