Hydrogen sulfide attenuates the inflammatory response in a mouse burn injury model

Zeng,Jianhong; Lin,Xianhe; Fan,Haipeng; Li,Changqing

doi:10.3892/mmr.2013.1610

Hydrogen sulfide attenuates the inflammatory response in a mouse burn injury model

Authors:
- Jianhong Zeng
- Xianhe Lin
- Haipeng Fan
- Changqing Li
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Affiliations: College of Pharmacy, Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Department of Cardiology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, Department of Anesthesiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250011, P.R. China, Department of Pharmacy, The Second Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing 400041, P.R. China
Published online on: August 1, 2013 https://doi.org/10.3892/mmr.2013.1610
Pages: 1204-1208

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Abstract

Hydrogen sulfide (H2S) is a naturally occurring gaseous transmitter, which is important in normal physiology and disease. In the present study, the involvement of H2S in the regulation of the immune response induced by burn injury was investigated in mice. Adult male C57BL/6 mice were subjected to burn injuries and treated with vehicle (0.9% sodium chloride, NaCl; 100 ml/kg body weight; subcutaneously, s.c.) or the H2S donor (sodium hydrosulfide, NaHS; 2 mg/kg body weight; s.c.). Compared with the controls, mice which received burn injuries exhibited a significant decrease in plasma H2S levels. Moreover, the levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑8 significantly increased, while IL‑10 levels were decreased, compared with that of the controls in the plasma of mice subjected to burn injuries. Myeloperoxidase (MPO) activity in the liver tissue of injured mice was also markedly higher compared with that of the control group. However, the administration of NaHS significantly decreased the levels of TNF‑α, IL‑6 and IL‑8 but increased the levels of IL‑10 in the plasma of mice subjected to burn injuries. In addition, the MPO activity was decreased by NaHS. These results suggested that H2S regulates the inflammatory response induced by burn injury by modulating the levels of TNF‑α, IL‑6, IL‑8 and IL‑10. Thus, it was proposed that the administration of the H2S donor, NaHS, may be a useful therapy against the exaggerated immune response that is associated with burn injury.

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