CpG oligodeoxynucleotide induces bone marrow precursor cells into myeloid-derived suppressor cells

Chen,Jie; Deng,Chengyu; Shi,Qingmin; Jiang,Jun; Zhang,Yongbo; Shan,Wei; Sun,Weimin

doi:10.3892/mmr.2013.1655

CpG oligodeoxynucleotide induces bone marrow precursor cells into myeloid-derived suppressor cells

Authors:
- Jie Chen
- Chengyu Deng
- Qingmin Shi
- Jun Jiang
- Yongbo Zhang
- Wei Shan
- Weimin Sun
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Affiliations: Department of Immunology, National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, P.R. China, Department of Centre for Disease Prevention and Control, Chengdu Military Region, Chengdu, Sichuan 610021, P.R. China, Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: August 27, 2013 https://doi.org/10.3892/mmr.2013.1655
Pages: 1149-1154

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Abstract

Dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) perform a number of functions in different immunological settings. In standard in vitro experiments, DCs are produced from mouse bone marrow (BM) cells in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. Our previous study demonstrated that BM precursor cells could differentiate into MDSCs when co-cultured with poly (I:C). In the present study, BM precursor cells cultured in GM-CSF and IL-4 were treated with CpG oligodeoxynucleotide (CpG ODN). We observed that Gr1+CD11b+ cells exhibiting MDSC functions accumulated in the co-culture system. A similar phenomenon was also observed in Listeria monocytogenes-infected mice. In conclusion, we demonstrated that prolonged CpG ODN stimulation could inhibit the development of DCs and induce the differentiation of BM precursor cells into MDSCs.

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