Differential expression of leukocyte β2 integrin signal transduction‑associated genes in patients with symptomatic pulmonary embolism
- Authors:
- Jin Yun
- Qianglin Duan
- Lemin Wang
- Wei Lv
- Zhu Gong
- Fan Yang
- Yanli Song
View Affiliations
Affiliations: Department of Cardiology, Tongji Hospital, Tongji University, Shanghai 200065, P.R. China, Department of Laboratory Medicine, Tongji Hospital, Tongji University, Shanghai 200065, P.R. China
- Published online on: November 7, 2013 https://doi.org/10.3892/mmr.2013.1780
-
Pages:
285-292
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Abstract
Whole human genome oligo microarrays were employed to systematically investigate the differential expression characteristics of associated mRNAs, which were found in the signal transduction pathway of β2 integrins in peripheral blood mononuclear cells (PBMCs) between patients with symptomatic pulmonary embolism (PE) and controls. A total of 20 cases of PE patients and twenty gender‑ and age‑matched controls were recruited for the study. Human cDNA microarray analysis was used to detect the differences in mRNA expression between the two groups and a random variance model corrected t‑test was used to analyze the statistical data. A total of 80 associated mRNAs were detected. The mRNA expression of chemokines, ligands, inside‑out and outside‑in signaling pathway‑associated proteins were upregulated significantly in the PE group, compared with the controls. In five subunit‑associated mRNAs, the mRNA expression of ITGAL, ITGAM, ITGAX and ITGB2, which encode for the subunits of αL, αM, αX and β2, were upregulated in the PE group and the differences, with the exception of ITGB2, were statistically significant (P<0.05). The mRNA expression of ITGAD was downregulated; however, there was no significant difference (P>0.05). The expression of Fgr mRNA was significantly downregulated (P<0.01). Thus, in PE patients, bilateral signal transduction pathways of β2 integrins in neutrophils and monocytes were activated, enhancing innate immunity.
View Figures![](/article_images/mmr/9/1/MMR-09-01-0285-g00.jpg) |
Figure 1
|
![](/article_images/mmr/9/1/MMR-09-01-0285-g07.jpg) |
Figure 2
|
![](/article_images/mmr/9/1/MMR-09-01-0285-g01.jpg) |
Figure 3
|
![](/article_images/mmr/9/1/MMR-09-01-0285-g02.jpg) |
Figure 4
|
![](/article_images/mmr/9/1/MMR-09-01-0285-g03.jpg) |
Figure 5
|
![](/article_images/mmr/9/1/MMR-09-01-0285-g04.jpg) |
Figure 6
|
![](/article_images/mmr/9/1/MMR-09-01-0285-g05.jpg) |
Figure 7
|
View References
1
|
Smeeth L, Cook C, Thomas S, Hall AJ,
Hubbard R and Vallance P: Risk of deep vein thrombosis and
pulmonary embolism after acute infection in a community setting.
Lancet. 367:1075–1079. 2006. View Article : Google Scholar : PubMed/NCBI
|
2
|
Haoming S, Lemin W, Zhu G, et al: T cell
mediated immune deficiency or compromise in patients with CTEPH. Am
J Respir Crit Care Med. 183:417–418. 2011. View Article : Google Scholar : PubMed/NCBI
|
3
|
Gong Z, Liang AB, Wang LM, et al: The
expression and significance of immunity associated genes mRNA in
patients with pulmonary embolism. Zhonghua Nei Ke Za Zhi.
48:666–669. 2009.(In Chinese).
|
4
|
Plow EF, Haas TA, Zhang L, Loftus J and
Smith JW: Ligand binding to integrins. J Biol Chem.
275:21785–21788. 2000. View Article : Google Scholar : PubMed/NCBI
|
5
|
Xie Y, Duan Q, Wang L, Gong Z, Wang Q,
Song H and Wang H: Genomic characteristics of adhesion molecules in
patients with symptomatic pulmonary embolism. Mol Med Rep.
6:585–590. 2012.PubMed/NCBI
|
6
|
Kiema T, Lad Y, Jiang P, et al: The
molecular basis of filamin binding to integrins and competition
with talin. Mol Cell. 21:337–347. 2006. View Article : Google Scholar : PubMed/NCBI
|
7
|
Schenkel AR, Mamdouh Z and Muller WA:
Locomotion of monocytes on endothelium is a critical step during
extravasation. Nat Immunol. 5:393–400. 2004. View Article : Google Scholar : PubMed/NCBI
|
8
|
Iezzi G, Karjalainen K and Lanzavecchia A:
The duration of antigenic stimulation determines the fate of naive
and effector T cells. Immunity. 8:89–95. 1998. View Article : Google Scholar : PubMed/NCBI
|
9
|
Perez OD, Mitchell D, Jager GC, et al:
Leukocyte functional antigen 1 lowers T cell activation thresholds
and signaling through cytohesin-1 and Jun-activating binding
protein 1. Nat Immunol. 4:1083–1092. 2003. View Article : Google Scholar : PubMed/NCBI
|
10
|
Ehlers MR: CR3: a general purpose
adhesion-recognition receptor essential for innate immunity.
Microbes Infect. 2:289–294. 2000. View Article : Google Scholar : PubMed/NCBI
|
11
|
Varga G, Balkow S, Wild MK, et al: Active
MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation. Blood.
109:661–669. 2007. View Article : Google Scholar : PubMed/NCBI
|
12
|
Han C, Jin J, Xu S, Liu H, Li N and Cao X:
Integrin CD11b negatively regulates TLR-triggered inflammatory
responses by activating Syk and promoting degradation of MyD88 and
TRIF via Cbl-b. Nat Immunol. 11:734–742. 2010. View Article : Google Scholar
|
13
|
Danilenko DM, Rossitto PV, Van der Vieren
M, Le Trong H, McDonough SP, Affolter VK and Moore PF: A novel
canine leukointegrin, alpha d beta 2, is expressed by specific
macrophage subpopulations in tissue and a minor CD8+
lymphocyte subpopulation in peripheral blood. J Immunol. 155:35–44.
1995.PubMed/NCBI
|
14
|
Andreas EM: Urokinase receptor surface
expression regulates monocyte adhesion in acute myocardial
infarction. Blood. 100:3611–3617. 2002. View Article : Google Scholar : PubMed/NCBI
|