Morphology, differentiation and adhesion molecule expression changes of bone marrow mesenchymal stem cells from acute myeloid leukemia patients
- Authors:
- Qiulei Chen
- Yan Yuan
- Tong Chen
-
View Affiliations
Affiliations: Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
- Published online on: November 11, 2013 https://doi.org/10.3892/mmr.2013.1789
-
Pages:
293-298
Metrics:
Total
Views: 0 (Spandidos Publications: | PMC Statistics:
)
Metrics:
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics:
)
This article is mentioned in:
Abstract
Bone marrow mesenchymal stem cells (BMSCs) have been identified as an important component of the hematopoietic stem cell (HSC) niche, which is essential for the maintenance of HSCs. HSC niche alternation has been considered to be the main cause of acute myeloid leukemia (AML). However, little is known with regard to BMSC alteration in AML patients. BMSCs were collected from 10 AML patients and 13 controls in order to examine the morphology, differentiation and adhesion molecule expression changes. It was observed that primary BMSCs from AML patients exhibited aberrant morphologies compared with those from the controls. Prior to adipogenic differentiation, the mRNA and protein levels of the lipid marker gene lipoprotein lipase, from the BMSCs of AML patients, were significantly higher. lipid drops were present early during differentiation in the BMSCs of AML patients and exhibited greater numbers later. Following adipogenic differentiation, the mRNA level of E-cadherin in the BMSCs of AML patients was significantly lower than that identified in the BMSCs of the control groups. Following osteogenic induction, the mRNA level of E-cadherin in the BMSCs of AML patients was significantly higher than in the controls. Therefore BMSCs from the AML patients exhibited irregular morphology, tendency to pre-differentiate to adipocytes and different adhesion molecule expression following differentiation. These differences may further our understanding of the HSC niche in the pathological condition.
View Figures |
Figure 1
|
 |
Figure 2
|
 |
Figure 3
|
 |
Figure 4
|
 |
Figure 5
|
 |
Figure 6
|
View References
|
1
|
Pittenger MF, Mackay AM, Beck SC, et al:
Multilineage potential of adult human mesenchymal stem cells.
Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI
|
|
2
|
Méndez-Ferrer S, Michurina TV, Ferraro F,
Mazloom AR, Macarthur BD, Lira SA, Scadden DT, Ma’ayan A,
Enikolopov GN and Frenette PS: Mesenchymal and haematopoietic stem
cells form a unique bone marrow niche. Nature. 466:829–834.
2010.PubMed/NCBI
|
|
3
|
Wang LD and Wagers AJ: Dynamic niches in
the origination and differentiation of haematopoietic stem cells.
Nat Rev Mol Cell Biol. 12:643–655. 2011. View Article : Google Scholar : PubMed/NCBI
|
|
4
|
Kiel MJ and Morrison SJ: Uncertainty in
the niches that maintain haematopoietic stem cells. Nat Rev
Immunol. 8:290–301. 2008. View
Article : Google Scholar : PubMed/NCBI
|
|
5
|
Steffen B, Müller-Tidow C, Schwäble J,
Berdel WE and Serve H: The molecular pathogenesis of acute myeloid
leukemia. Crit Rev Oncol Hematol. 56:195–221. 2005. View Article : Google Scholar : PubMed/NCBI
|
|
6
|
Calvi LM, Adams GB, Weibrecht KW, et al:
Osteoblastic cells regulate the haematopoietic stem cell niche.
Nature. 425:841–846. 2003. View Article : Google Scholar : PubMed/NCBI
|
|
7
|
Zhang J, Niu C, Ye L, et al:
Identification of the haematopoietic stem cell niche and control of
the niche size. Nature. 425:836–841. 2003. View Article : Google Scholar : PubMed/NCBI
|
|
8
|
Naveiras O, Nardi V, Wenzel PL, Hauschka
PV, Fahey F and Daley GQ: Bone-marrow adipocytes as negative
regulators of the haematopoietic microenvironment. Nature.
460:259–263. 2009. View Article : Google Scholar : PubMed/NCBI
|
|
9
|
Takeichi M: Cadherins: A molecular family
important in selective cell-cell adhesion. Annu Rev Biochem.
59:237–252. 1990. View Article : Google Scholar : PubMed/NCBI
|
|
10
|
Hosokawa K, Arai F, Yoshihara H, et al:
Cadherin based adhesion is a potential target for niche
manipulation to protect hematopoietic stem cells in adult bone
marrow. Cell Stem Cell. 6:194–198. 2010. View Article : Google Scholar : PubMed/NCBI
|
|
11
|
Hosokawa K, Arai F, Yoshihara H, et al:
Knockdown of N-cadherin suppresses the long-term engraftment of
hematopoietic stem cells. Blood. 116:554–563. 2010. View Article : Google Scholar : PubMed/NCBI
|
|
12
|
Kiel MJ, Radice GL and Morrison SJ: Lack
of evidence that hematopoietic stem cells depend on
N-cadherin-mediated adhesion to osteoblasts for their maintenance.
Cell Stem Cell. 1:204–217. 2007. View Article : Google Scholar : PubMed/NCBI
|
|
13
|
Kiel MJ, Acar M, Radice GL and Morrison
SJ: Hematopoietic stem cells do not depend on N-cadherin to
regulate their maintenance. Cell Stem Cell. 4:170–179. 2009.
View Article : Google Scholar : PubMed/NCBI
|
|
14
|
Wetzler M, Kurzrock R, Estrov Z, Estey E
and Talpaz M: Cytokine expression in adherent layers from patients
with myelodysplastic syndrome and acute myelogenous leukemia.
Leukemia Res. 19:23–34. 1995. View Article : Google Scholar : PubMed/NCBI
|
