CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues

Xiao,Songshu; Zhou,Yanhong; Jiang,Jianfa; Yuan,Le; Xue,Min

doi:10.3892/mmr.2014.2010

CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues

Authors:
- Songshu Xiao
- Yanhong Zhou
- Jianfa Jiang
- Le Yuan
- Min Xue
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Affiliations: Department of Gynecology and Obstetrics, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Molecular Genetics Laboratory, Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China
Published online on: March 6, 2014 https://doi.org/10.3892/mmr.2014.2010
Pages: 1667-1674

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Abstract

Cervical carcinoma is the second most prevalent type of malignancy in females worldwide. The crucial etiological factors involved in the development of cervical carcinoma include infection with the papillomavirus, and the structural or functional mutation of oncogenes and tumor suppressor genes. CD44 refers to a multifunctional family of type I transmembrane proteins. These proteins have been implicated in numerous biological processes, including cell adhesion, cell migration and metastasis. The present study examined the differences in the expression levels of ATP‑binding cassette sub‑family G member 2, CD24, CD44, CD133, cytokeratin (CK) 14 and CK19 between cervical cancer tissues and corresponding normal non‑tumor tissues by flow cytometry. Then, the CD44+ or CD44‑ cells from cervical cancer tissues were sorted for identification and confirmation of differential expression by flow cytometry. The results demonstrated that the expression level of CD44 in cervical cancer tissues was higher than in the corresponding non‑tumor normal tissues (t=3.12; P=0.0102). Compared with the CD44‑ cells, the FOS‑like antigen 1 (Fra‑1), nestin, nuclear receptor subfamily 4, group A, member 2, OCT4 and p63 genes were highly expressed in CD44+ cells. The fold changes were 3.55, 3.55, 2.46, 2.87 and 2.56, respectively (P<0.05). However, BMI1 polycomb ring finger oncogene, ck5, tumor protein p53 and lactotransferrin genes exhibited low expression levels in CD44+ cells. It was verified by western blot analysis and flow cytometry that Fra‑1 was highly expressed in CD44+ cells. Fra‑1 was a potential target of miR‑19a and miR‑19b. The expression of miR‑19a and miR‑19b was downregulated by ~50% in CD44+ cells compared with CD44‑ cells. These findings suggested that CD44 dysregulated the activation of the Fra‑1 gene. The interaction of Fra‑1 and CD44 may therefore be important in cervical carcinoma.

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