Cartilage tissue engineering using PHBV and PHBV/Bioglass scaffolds

Zhou,Mingshu; Yu,Dong

doi:10.3892/mmr.2014.2145

Cartilage tissue engineering using PHBV and PHBV/Bioglass scaffolds

Authors:
- Mingshu Zhou
- Dong Yu
View Affiliations

Affiliations: Department of Plastic and Reconstructive Surgery, The Third Hospital Affiliated to Shandong Academy of Medical Sciences, Shandong Institute of Parasitic Diseases, Jining, Shandong 272033, P.R. China, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
Published online on: April 15, 2014 https://doi.org/10.3892/mmr.2014.2145
Pages: 508-514

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Scaffolds have an important role in cartilage tissue engineering. Poly(hydroxybutyrate‑co‑hydroxyvalerate) (PHBV) has been demonstrated to have potential as a scaffold for the three dimensional construction of engineered cartilage tissue. However, the poor hydrophilicity and mechanical strength associated with PHBV affects its clinical applications as a scaffold in cartilage tissue engineering. The incorporation of Bioglass (BG) into PHBV has been shown to improve the hydrophilicity and mechanical strength of PHBV matrices. Therefore, this study aimed to compare the properties of PHBV scaffolds and PHBV scaffolds containing 10% BG (w/w) (PHBV/10% BG) and to investigate the effects of these scaffolds on the properties of engineered cartilage in vivo. Rabbit auricular chondrocytes were seeded onto PHBV and PHBV/10% BG scaffolds. Differences in cartilage regeneration were compared between the neocartilage grown on the PHBV and the PHBV/10% BG scaffolds after 10 weeks of in vivo transplantation. The incorporation of BG into PHBV was observed to improve the hydrophilicity and compressive strength of the scaffold. Furthermore, after 10 weeks incubation in vivo, the cartilage‑like tissue formed using the PHBV/10% BG scaffolds was observed to be thicker, exhibit enhanced biomechanical properties and have a higher cartilage matrix content than that generated using the pure PHBV scaffolds. The results of this study demonstrate that the incorporation of BG into PHBV may generate composite scaffolds with improved properties for cartilage engineering.

View Figures

View References

1	Vacanti CA, Langer R, Schloo B and Vacanti JP: Synthetic polymers seeded with chondrocytes provide a template for new cartilage formation. Plast Reconstr Surg. 88:753–759. 1991. View Article : Google Scholar : PubMed/NCBI
2	Xue K, Zhu Y, Zhang Y, Chiang C, Zhou G and Liu K: Xenogeneic chondrocytes promote stable subcutaneous chondrogenesis of bone marrow-derived stromal cells. Int J Mol Med. 29:146–152. 2012.PubMed/NCBI
3	Zhu S, Zhang T, Sun C, Yu A, Qi B and Cheng H: Bone marrow mesenchymal stem cells combined with calcium alginate gel modified by hTGF-β1 for the construction of tissue-engineered cartilage in three-dimensional conditions. Exp Ther Med. 5:95–101. 2013.PubMed/NCBI
4	Gu Y, Zhu W, Hao Y, Lu L, Chen Y and Wang Y: Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model. Exp Ther Med. 3:293–298. 2012.PubMed/NCBI
5	Kuhne M, John T, El-Sayed K, et al: Characterization of auricular chondrocytes and auricular/articular chondrocyte co-cultures in terms of an application in articular cartilage repair. Int J Mol Med. 25:701–708. 2010.PubMed/NCBI
6	Schlegel W, Nürnberger S, Hombauer M, Albrecht C, Vécsei V and Marlovits S: Scaffold-dependent differentiation of human articular chondrocytes. Int J Mol Med. 22:691–699. 2008.PubMed/NCBI
7	Sun J, Wu J, Li H and Chang J: Macroporous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) matrices for cartilage tissue engineering. Eur Polym J. 41:2443–2449. 2005. View Article : Google Scholar
8	Köse GT, Korkusuz F, Ozkul A, et al: Tissue engineered cartilage on collagen and PHBV matrices. Biomaterials. 26:5187–5197. 2005.PubMed/NCBI
9	Lu HD, Cai DZ, Wu G, Wang K and Shi DH: Whole meniscus regeneration using polymer scaffolds loaded with fibrochondrocytes. Chin J Traumatol. 14:195–204. 2011.PubMed/NCBI
10	Wu J, Xue K, Li H, Sun J and Liu K: Improvement of PHBV scaffolds with Bioglass for cartilage tissue engineering. PLoS One. 8:e715632013. View Article : Google Scholar : PubMed/NCBI
11	Li H, Du R and Chang J: Fabrication, characterization, and in vitro degradation of composite scaffolds based on PHBV and bioactive glass. J Biomater Appl. 20:137–155. 2005. View Article : Google Scholar : PubMed/NCBI
12	Li H and Chang J: Fabrication and characterization of bioactive wollastonite/PHBV composite scaffolds. Biomaterials. 25:5473–5480. 2004. View Article : Google Scholar : PubMed/NCBI
13	Hou Q, Grijpma DW and Feijen J: Porous polymeric structures for tissue engineering prepared by a coagulation, compression moulding and salt leaching technique. Biomaterials. 24:1937–1947. 2003. View Article : Google Scholar
14	Zheng X, Yang F, Wang S, et al: Fabrication and cell affinity of biomimetic structured PLGA/articular cartilage ECM composite scaffold. J Mater Sci Mater Med. 22:693–704. 2011. View Article : Google Scholar : PubMed/NCBI
15	Whitney GA, Mera H, Weidenbecher M, Awadallah A, Mansour JM and Dennis JE: Methods for producing scaffold-free engineered cartilage sheets from auricular and articular chondrocyte cell sources and attachment to porous tantalum. Biores Open Access. 1:157–165. 2012.PubMed/NCBI
16	Li H, Zhai W and Chang J: In vitro biocompatibility assessment of PHBV/Wollastonite composites. J Mater Sci Mater Med. 19:67–73. 2008. View Article : Google Scholar : PubMed/NCBI
17	Enobakhare BO, Bader DL and Lee DA: Quantification of sulfated glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue. Anal Biochem. 243:189–191. 1996. View Article : Google Scholar : PubMed/NCBI
18	Reddy GK and Enwemeka CS: A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem. 29:225–229. 1996. View Article : Google Scholar : PubMed/NCBI
19	Carey J, Small CF and Pichora DR: In situ compressive properties of the glenoid labrum. J Biomed Mater Res. 51:711–716. 2000. View Article : Google Scholar : PubMed/NCBI
20	Xue K, Qi L, Zhou G and Liu K: A two-step method of constructing mature cartilage using bone marrow-derived mesenchymal stem cells. Cells Tissues Organs. 197:484–495. 2013. View Article : Google Scholar : PubMed/NCBI
21	Yang S, Leong KF, Du Z and Chua CK: The design of scaffolds for use in tissue engineering. Part I Traditional factors. Tissue Eng. 7:679–689. 2001. View Article : Google Scholar : PubMed/NCBI
22	Dhandayuthapani B, Yoshida Y, Maekawa T and Kumar DS: Polymeric scaffolds in tissue engineering application: A review. Int J of Polym Sci. 2011:1–19. 2011. View Article : Google Scholar
23	Surrao DC, Khan AA, McGregor AJ, Amsden BG and Waldman SD: Can microcarrier-expanded chondrocytes synthesize cartilaginous tissue in vitro? Tissue Eng Part A. 17:1959–1967. 2011. View Article : Google Scholar : PubMed/NCBI
24	Schuh E, Kramer J, Rohwedel J, et al: Effect of matrix elasticity on the maintenance of the chondrogenic phenotype. Tissue Eng Part A. 16:1281–1290. 2010. View Article : Google Scholar : PubMed/NCBI
25	Hambleton J, Schwartz Z, Khare A, et al: Culture surfaces coated with various implant materials affect chondrocyte growth and metabolism. J Orthop Res. 12:542–552. 1994. View Article : Google Scholar : PubMed/NCBI
26	Boyan BD, Hummert TW, Dean DD and Schwartz Z: Role of material surfaces in regulating bone and cartilage cell response. Biomaterials. 17:137–146. 1996. View Article : Google Scholar : PubMed/NCBI
27	Gugala Z and Gogolewski S: Differentiation, growth and activity of rat bone marrow stromal cells on resorbable poly(L/DL-lactide) membranes. Biomaterials. 25:2299–2307. 2004. View Article : Google Scholar : PubMed/NCBI
28	Lydon MJ, Minett TW and Tighe BJ: Cellular interactions with synthetic polymer surfaces in culture. Biomaterials. 6:396–402. 1985. View Article : Google Scholar : PubMed/NCBI
29	van Wachem PB, Beugeling T, Feijen J, Bantjes A, Detmers JP and van Aken WG: Interaction of cultured human endothelial cells with polymeric surfaces of different wettabilities. Biomaterials. 6:403–408. 1985.PubMed/NCBI
30	Li X, Liu KL, Wang M, et al: Improving hydrophilicity, mechanical properties and biocompatibility of poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate] through blending with poly[(R)-3-hydroxybutyrate]-alt-poly(ethylene oxide). Acta Biomater. 5:2002–2012. 2009.
31	Wang Y, Lu L, Zheng Y and Chen X: Improvement in hydrophilicity of PHBV films by plasma treatment. J Biomed Mater Res A. 76:589–595. 2006. View Article : Google Scholar : PubMed/NCBI
32	Yu DG, Lin WC, Lin CH and Yang MC: Cytocompatibility and antibacterial activity of a PHBV membrane with surface-immobilized water-soluble chitosan and chondroitin-6-sulfate. Macromol Biosci. 6:348–357. 2006. View Article : Google Scholar : PubMed/NCBI
33	Köse GT, Korkusuz F, Korkusuz P, Purali N, Ozkul A and Hasirci V: Bone generation on PHBV matrices: an in vitro study. Biomaterials. 24:4999–5007. 2003.PubMed/NCBI
34	Köse GT, Korkusuz F, Korkusuz P and Hasirci V: In vivo tissue engineering of bone using poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) and collagen scaffolds. Tissue Eng. 10:1234–1250. 2004.PubMed/NCBI
35	Bal BS, Rahaman MN, Jayabalan P, et al: In vivo outcomes of tissue-engineered osteochondral grafts. J Biomed Mater Res B Appl Biomater. 93:164–174. 2010.PubMed/NCBI
36	Wang B, Li J, Zhang J, et al: Thermo-mechanical properties of the composite made of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) and acetylated chitin nanocrystals. Carbohydr Polym. 95:100–106. 2013. View Article : Google Scholar : PubMed/NCBI
37	Wang A, Gan Y, Yu H, et al: Improvement of the cytocompatibility of electrospun poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate] mats by Ecoflex. J Biomed Mater Res A. 100:1505–1511. 2012.PubMed/NCBI
38	Rieppo J, Töyräs J, Nieminen MT, et al: Structure-function relationships in enzymatically modified articular cartilage. Cells Tissues Organs. 175:121–132. 2003. View Article : Google Scholar : PubMed/NCBI
39	Mikic B, Isenstein AL and Chhabra A: Mechanical modulation of cartilage structure and function during embryogenesis in the chick. Ann Biomed Eng. 32:18–25. 2004. View Article : Google Scholar : PubMed/NCBI
40	Bastiaansen-Jenniskens YM, Koevoet W, de Bart AC, et al: Contribution of collagen network features to functional properties of engineered cartilage. Osteoarthritis Cartilage. 16:359–366. 2008. View Article : Google Scholar : PubMed/NCBI