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Preparation and characterization of luteinising‑hormone releasing hormone nanoliposomal microbubbles specifically targeting ovarian cancer cells in vitro

  • Authors:
    • Jinyi Zhang
    • Sisun Liu
    • Yuanfang Zhu
    • Liping Zhang
    • Wenjuan Li
    • Fen Wang
    • Shuying Huang

  • View Affiliations

  • Published online on: May 6, 2014     https://doi.org/10.3892/mmr.2014.2211
  • Pages: 567-571
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Abstract

The aim of the present study was to prepare luteinizing‑hormone releasing hormone (LHRH) nanoliposomal microbubbles specifically targeting ovarian cancer cells. The lyophilization/sonication method was used to prepare non‑targeting nanoliposomal microbubbles (N‑N‑Mbs). Using the biotin‑avidin bridge method, conjugated LHRH antibodies to N‑N‑Mbs generated LHRH nanoliposomal microbubbles (LHRH‑N‑Mbs) specifically targeting ovarian cancer cells. The morphology and physicochemical properties of the microbubbles was detected using an optical microscope and zeta detector. The binding affinity between the secondary antibody and LHRH‑N‑Mbs or N‑N‑Mbs was determined by flow cytometry. The binding of LHRH‑N‑Mb to human ovarian cancer cells (OVCAR‑3) was detected by light microscopy. The rounded and uniformly distributed N‑N‑Mbs and LHRH‑N‑Mbs were successfully generated. The particle size ranged from 295‑468 nm with a mean of 360 nm for N‑N‑Mbs or 369‑618 nm with a mean of 508 nm for LHRH‑N‑Mbs. There was a significant difference in size between the two groups (P<0.05), although the surface potential of the two microbubbles remained the same (‑14.6 mV). Following being kept at room temperature for 14 days, no significant difference in the physicochemical properties of the LHRH‑N‑Mbs was detected compared with that of freshly prepared microbubbles. The secondary antibody binding rate of LHRH‑N‑Mbs and N‑N‑Mbs was 75.6 and 0.83%, respectively. Furthermore, the formation of a rosette‑like structure surrounding OVCAR‑3 cells was observed after the cells were incubated with LHRH‑N‑Mbs, whereas pre‑incubation with LHRH antibody blocked this rosette formation. In conclusion, LHRH‑N‑Mbs specifically targeting ovarian cancer cells were successfully prepared through biotin‑avidin mediation and the lyophilization/sonication method. The key feature of LHRH‑N‑Mbs is their small size, stability and high efficiency in targeting human OVCAR‑3 cells in vitro.
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July-2014
Volume 10 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 6.9
  • CiteScore Rank: Q2: #98/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.721
  • SCImago Journal Rank (SJR): 0.65
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Spandidos Publications style
Zhang J, Liu S, Zhu Y, Zhang L, Li W, Wang F and Huang S: Preparation and characterization of luteinising‑hormone releasing hormone nanoliposomal microbubbles specifically targeting ovarian cancer cells in vitro. Mol Med Rep 10: 567-571, 2014
APA
Zhang, J., Liu, S., Zhu, Y., Zhang, L., Li, W., Wang, F., & Huang, S. (2014). Preparation and characterization of luteinising‑hormone releasing hormone nanoliposomal microbubbles specifically targeting ovarian cancer cells in vitro. Molecular Medicine Reports, 10, 567-571. https://doi.org/10.3892/mmr.2014.2211
MLA
Zhang, J., Liu, S., Zhu, Y., Zhang, L., Li, W., Wang, F., Huang, S."Preparation and characterization of luteinising‑hormone releasing hormone nanoliposomal microbubbles specifically targeting ovarian cancer cells in vitro". Molecular Medicine Reports 10.1 (2014): 567-571.
Chicago
Zhang, J., Liu, S., Zhu, Y., Zhang, L., Li, W., Wang, F., Huang, S."Preparation and characterization of luteinising‑hormone releasing hormone nanoliposomal microbubbles specifically targeting ovarian cancer cells in vitro". Molecular Medicine Reports 10, no. 1 (2014): 567-571. https://doi.org/10.3892/mmr.2014.2211