O-GlcNAcylation is increased in prostate cancer tissues and enhances malignancy of prostate cancer cells

  • Authors:
    • Yuchao Gu
    • Jiangang Gao
    • Cuifang Han
    • Xinling Zhang
    • Haiyan Liu
    • Leina Ma
    • Xiaoqing Sun
    • Wengong Yu
  • View Affiliations

  • Published online on: May 26, 2014     https://doi.org/10.3892/mmr.2014.2269
  • Pages: 897-904
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Abstract

O-GlcNAc is an O-linked ?-N-acetylglucosamine moiety attached to the side-chain hydroxyl of a serine or threonine residue in numerous cytoplasmic and nuclear proteins. In this study, we detected the level of O-GlcNAc in prostate, liver and pancreatic cancer tissues, and found that the global O-GlcNAc modification also known as O-GlcNAcylation, is specifically increased in prostate cancer tissues compared to corresponding adjacent tissues. In addition, we found that global O-GlcNAcylation is increased in prostate cancer cells and not in benign prostatic hyperplasia (BPH) epithelial cells. O-GlcNAc enhanced the anchorage-independent growth and the migratory/invasive ability of prostate cancer cells. More importantly, we provide here, for the first time to the best of our knowledge, direct evidence that increased O-GlcNAcylation induces malignant transformation of nontumorigenic (BPH) cells. Furthermore, our study suggested that inhibiting the formation of the E-cadherin/catenin/cytoskeleton complex may underly the O-GlcNAc-induced prostate cancer progression. Overall, these findings indicated that O-GlcNAcylation is increased in prostate, but not in liver and pancreatic cancer tissues, and that O-GlcNAc can enhance the malignancy of prostate cancer cells.

Introduction

Numerous nuclear and cytoplasmic proteins have been found modified with O-β-N-acetylglucosamine (O-GlcNAc) at the hydroxyl moiety of serine or threonine residues. This moiety is dynamically added and removed by the O-GlcNAc transferase (OGT) and the O-GlcNAcase (OGA) enzymes, respectively (1). UDP-GlcNAc is the donor substrate of OGT, and is biosynthesized through the hexosamine pathway (HBP). The HBP flux is highly dependent on glucose and glutamine, with ~3–5% of total glucose entering this pathway (2). O-GlcNAc is a sensor of intracellular glucose metabolism, since the intracellular level of UDP-GlcNAc is the main regulatory factor for the activity of OGT (3). O-GlcNAcylation is altered in metabolism-associated diseases, such as type II diabetes (46), Alzheimer’s disease (7) and cancer (8). Therefore, abnormal O-GlcNAcylation may play critical roles in these pathological processes.

Aberrant metabolism is a hallmark of cancer. Most cancer cells show increased rates of glucose and glutamine utilization, up to 200-fold higher than those observed in the healthy cells they originate from, and predominantly produce energy through glycolysis followed by lactic acid fermentation (9,10). As a glycolytic pathway, HBP can enhance the level of UDP-GlcNAc, and thus, the activity of OGT, which may lead to an increase in the global O-GlcNAc level (summed over all proteins) in cancer cells. We have previously demonstrated that the global O-GlcNAc level is increased in breast, lung and colon cancer tissues as compared to respective adjacent tissues (11,12). In addition, other research groups have shown that O-GlcNAcylation is increased in breast, prostate and pancreatic cancer cell lines (1315). However, whether increased O-GlcNAcylation is universal in cancer is unknown.

In this study, we examined the O-GlcNAc level in prostate, pancreatic and liver cancer tissues, and the results indicated that global O-GlcNAcylation is specifically increased in prostate cancer tissues compared to corresponding adjacent tissues. Furthermore, we investigated the roles of O-GlcNAc in prostate cancer cell proliferation and invasion, along with the underlying mechanism.

Materials and methods

Specimens and ethics

Liver, pancreatic and prostate cancer tissue microarrays (TMAs; OD-CT-DgLiv03-002, OD-CT-DgPan03-002, and OD-CT-UrPrt03-001 respectively) were purchased from Shanghai Outdo Biotech Co. (Shanghai, China). The liver cancer tissue TMA was constructed with 31 formalin-fixed, paraffin-embedded hepatocellular carcinoma tissues and their corresponding adjacent liver tissues, the pancreatic cancer tissue TMA was constructed with 31 formalin-fixed, paraffin-embedded pancreatic ductal adenocarcinoma tissues and their corresponding adjacent pancreatic tissues, and the prostate cancer tissue TMA was constructed with 29 formalin-fixed, paraffin-embedded prostate cancer tissues and their corresponding adjacent benign prostatic hyperplasia (BPH) tissues. In addition, 26 prostate cancer tissues and 19 BPH tissues, which were fixed with formalin and embedded with paraffin, were obtained from the Department of Urology Surgery, Qingdao Municipal Hospital (QMH). The tissues were cut into 6-μm-thick sections and mounted on glass slides. Written informed consent was acquired from all patients and/or guardians for the use of their tissue samples. The present study was reviewed and approved by the Research Ethics Committee of Qingdao Municipal Hospital (Qingdao, China).

Immunohistochemistry

Immunohistochemistry was performed on the specimens using the DAKO Liquid DAB Substrate Chromogen system (Dako North America, Inc., Carpinteria, CA, USA) and a monoclonal mouse antibody targeting O-GlcNAc (RL2; Affinity BioReagents, Inc., Golden, CO, USA), used at a 1:200 dilution. The Fromowitz standard was used to semi-quantitatively assess the staining intensity of O-GlcNAc (16,17).

Cell cultures

The human BPH-1 line is representative of benign prostatic hyperplasia, while the PC3 and DU145 prostate cancer cell lines are representative of the earlier type-I ADI prostate cancers. All cell lines were maintained in RPMI-1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Corning Cellgro, Manassas, VA, USA). To increase the O-GlcNAc level, cells were treated for 24 h or the indicated time period with 10 μM thiamet-G, a selective OGA inhibitor, synthesized as previously described (18).

Immunoblotting (IB)

Cells were lysed in lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP40, 1 mM EDTA, 1 mM Na3VO4, 10 mM NaF] containing a protease inhibitor cocktail (Roche Applied Science, Mannheim, Germany), 50 mM GlcNAc and 5 μM PUGNAc (both from Toronto Research Chemicals Inc., North York, ON, Canada). Protein samples (30 μg) were separated by 7.5% dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and transferred to Immobilon-P membranes (Millipore, Billerica, MA, USA). Antibodies targeting O-GlcNAc (CTD110.6; Abcam, Cambridge, UK), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), OGT (F-12) and OGA (all from Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), were used along with the Amersham ECL Prime (GE Healthcare, Buckinghamshire, UK).

Soft agar assay

The soft agar assay was performed as described in (19). Briefly, cells (1×104) were suspended in 1 ml top agar medium (RPMI-1640 medium supplemented with 10% FBS in 0.4% agar). The cell suspension was then overlaid onto 1.5 ml bottom agar medium (the corresponding medium in 0.8% agar) in 6-well tissue culture plates in triplicate. Fresh medium was added to the plates every 3 days as a feeder layer. On the 5th day, images were acquired from 6 random fields of the colonies at ×40 magnification.

Cell invasion and migration assay

The cell invasion and migration assays were performed as described in (19). Transwell chambers (6.5 mm; Corning, New York, NY, USA) with 8 μm-pore membranes were used for the cell migration assay. The lower chamber was filled with RPMI-1640 medium (supplemented with 20% FBS) and thiamet-G, except for control samples. Cells (5×104) were suspended in 100 μl upper medium (RPMI-1640 medium with 1% FBS) and placed into the upper chamber with or without thiamet-G. After 16 h, the number of cells detected by crystal violet staining on the undersurface of the polycarbonate membranes was visually scored in five random fields at ×100 magnification using a light microscope.

The invasion assay was performed using the same procedure as the migration assay, except that the upper surface of the membrane was covered with 70 μl of 1 mg/ml Matrigel (BD Biosciences, San José, CA, USA) and the incubation time was prolonged to 24 h.

RNA isolation and quantitative reverse transcription (RT)-PCR

Total RNA was isolated from the cells using an RNA isolation kit (BioTeke Corporation, Beijing, China), and reverse transcription was carried out using the M-MLV reverse transcriptase (Takara Biotechnology Co., Ltd., Dalian, China). Quantitative PCR assays on the resulting cDNA products were carried out in triplicate using the SYBR® Green PCR Master mix (Applied Biosystems/Life Technologies, Carlsbad, CA, USA) on a 7500 Real-time PCR system (Applied Biosystems/Life Technologies). The levels of the tested genes were normalized to that of the endogenous control gene GAPDH. The primers for amplification used were the following: human E-cadherin forward, 5′-GCC ACC CTG GCT TTG ACG-3′, and reverse, 5′-CCA TCT GTG CCC ACT TTG AAT C-3′; human GAPDH forward, 5′-CAG GGC TGC TTT TAA CTC TGG T-3′, and reverse 5′-CCT GGA AGA TGG TGA TGG GAT-3′.

Extraction of cytoskeleton-binding proteins

To separate cytoskeleton-binding proteins from other cell constituents, BPH-1 and DU145 cells were washed twice with phosphate-buffered saline, lysed on ice for 15 min in Triton X-100 lysis buffer [300 mM sucrose, 10 mM PIPES (pH 6.8), 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, 0.1 mg/ml DNase, 0.1 mg/ml RNase, 1.2 mM phenylmethanesulfonyl fluoride and protease inhibitor mix cocktail], passed through a 26 gauge needle four times, then subjected to centrifugation at 48,000 × g for 10 min at 4°C. The pellet was solubilized in loading buffer containing 2% SDS, and the supernatant and pellet fractions were loaded onto separate gels and processed as previously described (20).

Statistical analysis

Data were analyzed by Student’s t-tests using the SPSS 11.0 software (IBM, Armonk, NY, USA). P<0.05 was considered to indicate statistically significant differences. Data were expressed as the mean ± SD.

Results

The O-GlcNAc is increased in prostate cancer tissues

To investigate whether increased O-GlcNAcylation is universal in cancer, TMAs were used to detect the O-GlcNAc level in 31 hepatocellular carcinoma tissues (liver cancer), 31 pancreatic ductal adenocarcinoma tissues (pancreatic cancer), 29 prostate cancer tissues and their corresponding adjacent BPH tissues by immunohistochemistry, using an antibody targeting O-GlcNAc (RL2). Representative stained tissue sections are shown in the left and middle panels of Fig. 1. The intensity of O-GlcNAc immunostaining was analyzed by the Fromowitz standard. The results indicated that O-GlcNAcylation is increased in prostate cancer tissues as compared to the adjacent tissues; however, there was no significant difference between the liver/pancreatic cancer tissues and their corresponding adjacent tissues. To confirm the results observed in the prostate cancer tissues, 26 additional prostate cancer tissues and 19 BPH tissues were collected and assayed for O-GlcNAc by immunohistochemistry. In accordance with the results from the TMA samples, the level of O-GlcNAc was increased in prostate cancer tissues as compared to BPH tissues, and statistical analysis on the combined dataset from TMAs and collected tissues showed that this increase is significant (Fig. 1C, right panel). These results suggested that O-GlcNAc may be a good molecular marker of prostate cancer and may play important roles in prostate cancer progression.

O-GlcNAc and OGT levels are increased in prostate cancer cells

To investigate whether O-GlcNAcylation is increased in all types of prostate cancer compared to BPH cells, we detected the global O-GlcNAc level in PC3, DU145 and BPH-1 cells by IB using the CTD110.6 antibody. Comparison of the global protein expression patterns in BPH-1, PC3 and DU145 cells by SDS-PAGE indicated that there is no obvious difference between these three cell lines (Fig. 2A). However, as shown in Fig. 2B and C, where immunoblots were treated and analyzed by ECL, the global O-GlcNAc level was significantly increased in the PC3 and DU145 compared to the BPH-1 cells, especially the staining intensity of the high molecular weight bands (marked with arrows, Fig. 2B); however, the intensity of the lower molecular weight bands (marked with stars, Fig. 2B) was weaker in the prostate cancer cell lines compared to the BPH-1 line.

Although there is only one OGT-encoding gene in mammalian genomes, alternative promoter usage and/or alternative splicing produce three OGT isoforms, which differ in the length of their amino-terminal tetratricopeptide repeats (TPRs) (21). The nucleocytoplasmic OGT (ncOGT) contains 13 TPRs, the mitochondrial OGT (mOGT) contains a mitochondrial-targeting sequence at its N-terminus and nine TPRs, and the shortest OGT isoform (sOGT) contains only two TPRs. Previous studies have indicated that the expression of OGT is increased in certain cancer types, but the OGT isoforms were not discriminated in these reports (11,12,14,22). Here, we detected by IB the expression of OGT using specific antibodies that recognize all three OGT isoforms, and the results showed that ncOGT and mOGT, but not sOGT, are detectable in all tested cell lines (Fig. 2B). The expression of ncOGT was not significantly different between the prostate cancer and BPH cell lines, but the expression of mOGT was markedly decreased in prostate cancer cells (Fig. 2B and D). To our knowledge, this is the first report on decreased expression of the pro-apoptotic protein mOGT (21,23) in cancer cells.

We also examined the expression of OGA in PC3, DU145 and BPH-1 cells, and the results indicated that the expression of OGA is increased in DU145 cells (Fig. 2B). Considering that the level of O-GlcNAc in DU145 cells was found increased, the increased expression of OGA may be due to feedback regulation.

O-GlcNAc enhances the anchorage-independent growth of BPH-1 and prostate cancer cells

To determine whether O-GlcNAc is involved in prostate cancer malignancy, a highly potent and selective O-GlcNAcase inhibitor (thiamet-G) was used to increase the O-GlcNAc level in BPH-1, PC3 and DU145 cells (Fig. 3).

To investigate whether O-GlcNAc affects the proliferation and anchorage-independent growth of prostate cancer and BPH-1 cells, soft agar colony assays were performed. Thiamet-G treatment did not significantly affect the proliferation of the cells (MTT assay, data not shown). Soft agar colony assays showed that in the presence of thiamet-G, BPH-1, DU145 and PC3 cells form colonies at days 5–7 (Fig. 4); however, without thiamet-G, DU145 and PC3 cells were able to form colonies only two weeks later, while BPH-1 cells were not able to form colonies (data not shown).

Thiamet-G treatment not only enhanced colony formation in the prostate cancer cell lines DU145 and PC3, but further endowed BPH-1 cells with the ability to form colonies. These results indicated that O-GlcNAc is important for the anchorage-independent growth of prostate cancer cells, suggesting that increased O-GlcNAcylation may be sufficient for the malignant transformation of BPH cells.

O-GlcNAc enhances the invasion of prostate cancer cells

To investigate whether O-GlcNAc affects the invasive ability of prostate cancer cells, invasion assays were performed. As shown in Fig. 5, the invasive ability of DU145 (Fig. 5B) and PC3 cells (Fig. 5C) was significantly enhanced by thiamet-G treatment. However, neither BPH-1 nor the thiamet-G-treated BPH-1 cells showed invasive ability in our assays (data not shown); we thus next investigated the role of O-GlcNAc in BPH-1 migration. The results showed that O-GlcNAc markedly enhanced migration in BPH-1 cells (Fig. 5A). Overall, these results suggested that O-GlcNAcylation may play important roles in prostate cancer metastasis.

O-GlcNAc inhibits the formation of the E-cadherin/catenin complex

E-cadherin-mediated cel-cell adhesion is generally reduced in human cancers, and loss of E-cadherin is associated with tumor invasiveness, metastatic dissemination, and poor patient prognosis (24). In addition, Snail1 was reported to be stabilized by O-GlcNAcylation and thus, inhibit the transcription of E-cadherin (25). We therefore investigated whether O-GlcNAc can affect the expression of E-cadherin in prostate cancer cells. BPH-1, PC3 and DU145 cells were treated with thiamet-G for 24 h and the mRNA level of E-cadherin was detected by quantitative RT-PCR. The results indicated that the elevation of O-GlcNAc does not affect the transcription of E-cadherin in any of the three cell lines (Fig. 6A). The mRNA level of E-cadherin was lower in DU145 and PC3 compared to BPH-1 cells.

We previously reported that increased O-GlcNAcylation reduces the level of the cell surface protein E-cadherin, which has the potential to enhance cell invasion and metastasis in breast cancer cells (12). The binding of the E-cadherin/catenin complex to the cytoskeleton is essential for strong cell-cell adhesion (26), and we demonstrated that an increase in the O-GlcNAc level inhibits the formation of the E-cadherin/catenin/cytoskeleton complex (12). Here, the E-cadherin/catenin/cytoskeleton complex was isolated from BPH-1 and DU145 cell extracts in the Triton X-100-insoluble fraction. The levels of the cytoskeleton-associated proteins E-cadherin and p120 were reduced in the thiamet-G-treated BPH-1 (Fig. 6B and C) and DU145 (Fig. 6B and D) cells, suggesting that the reduction in the level of the cell surface protein E-cadherin may cause the enhanced migratory and invasive ability of prostate cancer cells.

Discussion

In previous studies, we analyzed histological sections from breast, lung and colon tumors and demonstrated that the O-GlcNAc level is increased in these tissues compared to matched adjacent tissues (11,12). Similarly, in patients with chronic lymphocytic leukaemia, the O-GlcNAc level was increased when compared to healthy lymphocytes (27). Similar results were also obtained in breast (14), prostate (13) and pancreatic cancer cell lines (15) by IB analysis. A most likely reason for the elevation of O-GlcNAcylation in cancer tissues/cells is the increased rate of glucose utilization, which may enhance the HBP pathway, and thus increase the concentration of UDP-GlcNAc in cancer cells. In line with this hypothesis, it has been reported that the concentration of UDP-GlcNAc is increased in chronic lymphocytic leukemia cells (27). Based on all the above, we argue that the increase in O-GlcNAc may be a universal event in different types of cancer that show alterations in glucose metabolic pathways (Warburg effect). However, we found that the levels of O-GlcNAc are not increased in liver and pancreatic cancer tissues. Two explanations for this contradictory result may be postulated: first, there is no report on the systematic comparison of the concentration of UDP-GlcNAc in solid cancer tissues or cells and their healthy counterparts. Therefore, we can not confirm whether UDP-GlcNAc is increased in different types of cancer. Second, a previous study reported that the apparent Km (Kmapp) values of OGT for UDP-GlcNAc vary depending on the protein substrate, suggesting that certain OGT substrates are nutrient-responsive, whereas others are constitutively modified (28). The constitutively modified proteins are more easily modified by O-GlcNAc, and thus have a higher O-GlcNAc stoichiometry; these proteins may be the main contributors of the increased global O-GlcNAc level observed by immunohistochemistry in cancer tissues/cells. In conclusion, the increase in global O-GlcNAcylation may not be a suitable marker for cancer; it may be more relevant to consider O-GlcNAcylation in specific proteins.

In contrast to our results, a recent report indicated that O-GlcNAcylation is globally increased in pancreatic ductal adenocarcinoma cell lines compared to non-transformed human pancreatic duct epithelial cells. In addition, the study also reported that pancreatic cancer tissue exhibits increased O-GlcNAc staining compared to healthy pancreatic ducts (15). However, this study was restricted to a limited number and type of cells and only used one tissue specimen. A potential explanation for the different results with our study may be the screening of different tissues.

Zhu et al compared the O-GlcNAc levels of forty hepatocellular carcinoma (HCC) tissues to ten healthy liver tissues by immunohistochemistry analysis. The authors found that global O-GlcNAcylation is significantly increased in HCC tissues compared to healthy liver tissues (29). By contrast, our study did not find a significant difference in the global O-GlcNAc level between hepatocellular carcinoma tissues and their matched adjacent tissues. One reason for the contradiction may be the fact that liver cancer adjacent tissues used in our study were chronic hepatitis, but not healthy liver tissues. Liver tissue inflammation may cause the deregulation in O-GlcNAcylation.

In this study, we found that global O-GlcNAc levels are increased in prostate cancer tissues compared to the BPH tissues, which was consistent with our previous finding that O-GlcNAc levels are increased in prostate cancer cell lines compared to the healthy prostate epithelium. As discussed above, the increase in the UDP-GlcNAc level may be the main cause for the upregulation of global O-GlcNAcylation in prostate cancer. However, another study showed that the increased expression of OGT may be an additionally important positive regulator of O-GlcNAcylation in prostate cancer (22). In this study, we detected the global O-GlcNAc and OGT levels in BPH-1, PC3 and DU145 cells. Although the global O-GlcNAc level was slightly increased in prostate cancer cells, the O-GlcNAc staining intensities were not homogeneously increased in all bands: the intensities of some O-GlcNAc-stained bands from prostate cancer cells were increased, while the intensities of other bands were decreased compared to BPH-1 cells. This might be explained by the different target protein preference of OGT in prostate cancer and BPH-1 cells.

Our study also demonstrated that O-GlcNAc can enhance the malignancy of several cancer types, and that the OGT level is increased in certain cancer cell lines. However, we found that the expression of mOGT is decreased in prostate cancer cells compared to BPH-1 cells. Considering that mOGT has pro-apoptotic roles, the reduced expression of mOGT may enhance the survival and anti-apoptotic abilities of prostate cancer cells. The roles of mOGT in cancer formation and progression need to be further investigated.

The human prostatic epithelial cell line BPH-1, derived from BPH tissue and immortalized with the SV40 large T antigen, is normally nontumorigenic in nude mice (30) and can not form colonies in the soft agar transformation assay. BPH-1 can be induced to form tumors by various factors, such as association with a tumorigenic stromal microenvironment, treatment with hormonal carcinogens or by oncogene transformation (31,32). In this study, we found that the increase in O-GlcNAcylation by thiamet-G induced anchorage-independent growth and migration in BPH-1 cells, which is the first direct evidence that O-GlcNAc can induce malignant transformation in bening cells. It is notable that thiamet-G treatment induced colony formation in BPH-1, PC3 and DU145 cells on the 4th-7th day during the soft agar assay, while two weeks were required for colony formation in the non-treated PC3 and DU145 cells (data not shown). Moreover, we found that thiamet-G treatment enhances invasion in PC3 and DU145 cells. Consistent with our results, Lynch et al reported that the OGT knock-down in prostate cancer cells inhibits anchorage-independent cell growth, cell invasion and angiogenesis (13). Based on these results, we propose that O-GlcNAc is involved in all steps of prostate cancer formation and progression.

E-cadherin is a well-known cell-cell adhesion molecule, which acts as a suppressor of cancer cell invasion; its expression is downregulated in numerous types of advanced human cancer. There have been some reports that provided evidence on the regulation of E-cadherin by O-GlcNAc. For example, O-GlcNAc inhibited the transcription of E-cadherin by modifying and stabilizing Snail1 (25); our group also reported that the increase in the O-GlcNAc level can inhibit the formation of the E-cadherin/catenin/cytoskeleton complex, and thus induce invasion and metastasis in breast cancer cells (12). In the present study, we found that the increase in the O-GlcNAc level does not affect the mRNA level of E-cadherin in BPH-1, PC3 and DU145 cells, but that O-GlcNAc inhibited the formation of the E-cadherin/catenin/cytoskeleton complex. Therefore, regulation of E-cadherin might constitute the mechanism underlying the induction of prostate cancer invasion. However, the invasive ability of PC3 cells, in which E-cadherin is expressed at low levels, was also significantly enhanced in our study. Furthermore, it was reported that reducing the expression of OGT leads to increased degradation of the FoxM1 protein, and thus regulates the progression of breast and prostate cancer cells (13). Moreover, there are numerous proteins related to tumor progression that are modified and regulated by O-GlcNAc (8). Overall, previous reports and present findings suggest that the mechanisms involved in the roles of O-GlcNAc in cancer progression may be multidimensional and/or highly context-dependent.

In summary, we showed that global O-GlcNAcylation is increased in prostate, but not liver and pancreatic cancer tissues compared to their corresponding adjacent tissues, and that the expression of mOGT, but not ncOGT, is decreased in prostate cancer cell lines; moreover, we found that O-GlcNAc can enhance the malignancy of prostate cancer cells, at least partially through inhibition of the formation of the E-cadherin/catenin/cytoskeleton complex.

Acknowledgements

We would like to thank Dr Xinzhi Lu, Feng Han and Qianhong Gong for numerous discussions. This work was supported by the National Natural Science Foundation of China (grant nos. 81101505, 81172013 and 81272264) and the Open Research Fund Program of the Key Laboratory of Marine Drugs [grant no. KLMD(OUC)201308].

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August-2014
Volume 10 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Gu Y, Gao J, Han C, Zhang X, Liu H, Ma L, Sun X and Yu W: O-GlcNAcylation is increased in prostate cancer tissues and enhances malignancy of prostate cancer cells. Mol Med Rep 10: 897-904, 2014.
APA
Gu, Y., Gao, J., Han, C., Zhang, X., Liu, H., Ma, L. ... Yu, W. (2014). O-GlcNAcylation is increased in prostate cancer tissues and enhances malignancy of prostate cancer cells. Molecular Medicine Reports, 10, 897-904. https://doi.org/10.3892/mmr.2014.2269
MLA
Gu, Y., Gao, J., Han, C., Zhang, X., Liu, H., Ma, L., Sun, X., Yu, W."O-GlcNAcylation is increased in prostate cancer tissues and enhances malignancy of prostate cancer cells". Molecular Medicine Reports 10.2 (2014): 897-904.
Chicago
Gu, Y., Gao, J., Han, C., Zhang, X., Liu, H., Ma, L., Sun, X., Yu, W."O-GlcNAcylation is increased in prostate cancer tissues and enhances malignancy of prostate cancer cells". Molecular Medicine Reports 10, no. 2 (2014): 897-904. https://doi.org/10.3892/mmr.2014.2269