Human breast adipose‑derived stem cells: Characterization and differentiation into mammary gland‑like epithelial cells promoted by autologous activated platelet‑rich plasma

  • Authors:
    • Shi-En Cui
    • Hong-Mian Li
    • Da-Lie Liu
    • Hua Nan
    • Kun-Ming  Xu
    • Pei-Ran Zhao
    • Shuang-Wu Liang
  • View Affiliations

  • Published online on: May 28, 2014     https://doi.org/10.3892/mmr.2014.2280
  • Pages: 605-614
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Abstract

Human adipose‑derived stem cells (ASCs) isolated from various body sites have been widely investigated in basic and clinical studies. However, ASCs derived from human breast tissue (hbASCs) have not been extensively investigated. In order to expand our understanding of hbASCs and examine their potential applications in stem cell research and cell‑based therapy, hbASCs were isolated from discarded surgical fat tissue following reduction mammoplasty and a comprehensive characterization of these hbASCs was performed, including analysis of their cellular morphology, growth features, cell surface protein markers and multilineage differentiation capacity. These hbASCs expressed cluster of differentiation (CD)44, CD49d, CD90 and CD105, but did not express CD31 and CD34. Subsequently, the hbASCs were differentiated into adipocytes, osteocytes and chondrocytes in vitro. In order to examine the potential applications of hbASCs in breast reconstruction, an approach to promote in vitro differentiation of hbASCs into mammary gland‑like epithelial cells (MGECs) was developed using activated autologous platelet‑rich plasma (PRP). A proliferation phase and a subsequent morphological conversion phase were observed during this differentiation process. PRP significantly promoted the growth of hbASCs in the proliferation phase and increased the eventual conversion rate of hbASCs into MGECs. Thus, to the best of our knowledge, the present study provided the first comprehensive characterization of hbASCs and validated their multipotency. Furthermore, it was revealed that activated autologous PRP was able to enhance the differentiation efficiency of hbASCs into MGECs. The present study and other studies of hbASCs may aid the development of improved breast reconstruction strategies.
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August-2014
Volume 10 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Cui S, Li H, Liu D, Nan H, Xu K, Zhao P and Liang S: Human breast adipose‑derived stem cells: Characterization and differentiation into mammary gland‑like epithelial cells promoted by autologous activated platelet‑rich plasma. Mol Med Rep 10: 605-614, 2014.
APA
Cui, S., Li, H., Liu, D., Nan, H., Xu, K., Zhao, P., & Liang, S. (2014). Human breast adipose‑derived stem cells: Characterization and differentiation into mammary gland‑like epithelial cells promoted by autologous activated platelet‑rich plasma. Molecular Medicine Reports, 10, 605-614. https://doi.org/10.3892/mmr.2014.2280
MLA
Cui, S., Li, H., Liu, D., Nan, H., Xu, K., Zhao, P., Liang, S."Human breast adipose‑derived stem cells: Characterization and differentiation into mammary gland‑like epithelial cells promoted by autologous activated platelet‑rich plasma". Molecular Medicine Reports 10.2 (2014): 605-614.
Chicago
Cui, S., Li, H., Liu, D., Nan, H., Xu, K., Zhao, P., Liang, S."Human breast adipose‑derived stem cells: Characterization and differentiation into mammary gland‑like epithelial cells promoted by autologous activated platelet‑rich plasma". Molecular Medicine Reports 10, no. 2 (2014): 605-614. https://doi.org/10.3892/mmr.2014.2280