Upregulation of heat shock factor 1 transcription activity is associated with hepatocellular carcinoma progression

Li,Shulian; Ma,Wanli; Fei,Teng; Lou,Qiang; Zhang,Yaqin; Cui,Xiukun; Qin,Xiaoming; Zhang,Jun; Liu,Guangchao; Dong,Zheng; Ma,Yuanfang; Song,Zhengshun; Hu,Yanzhong

doi:10.3892/mmr.2014.2547

Upregulation of heat shock factor 1 transcription activity is associated with hepatocellular carcinoma progression

Authors:
- Shulian Li
- Wanli Ma
- Teng Fei
- Qiang Lou
- Yaqin Zhang
- Xiukun Cui
- Xiaoming Qin
- Jun Zhang
- Guangchao Liu
- Zheng Dong
- Yuanfang Ma
- Zhengshun Song
- Yanzhong Hu
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Affiliations: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China, Department of Surgery, Huaihe Hospital Affiliated to Henan University, Kaifeng, Henan 475004, P.R. China, Department of Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai 20072, P.R. China, Department of Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai 20072, P.R. China
Published online on: September 8, 2014 https://doi.org/10.3892/mmr.2014.2547
Pages: 2313-2321
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Heat shock factor 1 (HSF1) is associated with tissue‑specific tumorigenesis in a number of mouse models, and has been used a as prognostic marker of cancer types, including breast and prostatic cancer. However, its role in human hepatocellular carcinoma (HCC) is not well understood. Using immunoblotting and immunohistochemical staining, it was identified that HSF1 and its serine (S) 326 phosphorylation, a biomarker of HSF1 activation, are significantly upregulated in human HCC tissues and HCC cell lines compared with their normal counterparts. Cohort analyses indicated that upregulation of the expression of HSF1 and its phospho‑S326 is significantly correlated with HCC progression, invasion and patient survival prognosis (P<0.001); however, not in the presence of a hepatitis B virus infection and the expression of alpha-fetoprotein and carcinoembryonic antigen. Knockdown of HSF1 with shRNA induced the protein expression of tumor suppressor retinoblastoma protein, resulting in attenuated plc/prf5 cell growth and colony formation in vitro. Taken together, these data markedly support that HSF1 is a potential prognostic marker and therapeutic target for the treatment of HCC.

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