Protein‑protein interaction network and mechanism analysis in ischemic stroke

Quan,Zhe; Quan,Yuan; Wei,Bo; Fang,Dening; Yu,Weidong; Jia,Hao; Quan,Wei; Liu,Yuguang; Wang,Qihong

doi:10.3892/mmr.2014.2696

Protein‑protein interaction network and mechanism analysis in ischemic stroke

Authors:
- Zhe Quan
- Yuan Quan
- Bo Wei
- Dening Fang
- Weidong Yu
- Hao Jia
- Wei Quan
- Yuguang Liu
- Qihong Wang
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Affiliations: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan, Shandong 250000, P.R. China, Norman Bethune Medical School of Jilin University, Changchun, Jilin 130000, P.R. China, Department of Neurosurgery, The Third Affiliated Hospital (China‑Japan Union Hospital) of Jilin University, Changchun, Jilin 130031, P.R. China, Department of Gastroenterology, Shanghai No. 8 Hospital, Shanghai 200235, P.R. China, Department of Emergency Medicine, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110000, P.R. China, Department of Infection, HuiNanXian Hospital, Huinan, Jilin 135100, P.R. China, Department of Neurosurgery, Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China
Published online on: October 17, 2014 https://doi.org/10.3892/mmr.2014.2696
Pages: 29-36
Copyright: © Quan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Ischemic stroke is a leading cause of mortality and permanent disability, with enormous financial repercussions on health systems worldwide. Ischemic brain injury results from a complex sequence of pathophysiological events that evolve over time. In order to examine the molecular mechanisms underlying middle cerebral artery occlusion (MCAO)‑induced ischemic stroke, the GSE35338 affymetrix microarray data was obtained from the Gene Expression Omnibus database and the differentially expressed genes (DEGs) between samples from patients with MCAO‑induced ischemic stroke and sham controls at various time points were identified. Furthermore, protein‑protein interaction (PPI) networks were constructed by mapping the DEGs into PPI data to identify the pathways that these DEGS are involved in. The results revealed that the expression of 438 DEGs, which are mainly involved in cell death, oxidant reduction, cell cycle and cell‑cell signaling, were altered in MCAO samples. The nodes of CXC motif chemokine 10 (CXCL10) and interleukin‑6 (IL‑6) were large, with degrees of >20. In conclusion, the results suggest that CXCL10 and IL‑6 have important roles in the occurrence and progression of MCAO‑induced ischemic stroke.

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