Increased immunoreactivity of c‑Fos in the spinal cord of the aged mouse and dog

Ahn,Ji  Hyeon; Shin,Myoung  Chul; Park,Joon  Ha; Kim,In  Hye; Lee,Jae‑Chul; Yan,Bing  Chun; Hwang,In  Koo; Moon,Seung  Myung; Ahn,Ji  Yun; Ohk,Taek  Geun; Lee,Tae  Hun; Cho,Jun  Hwi; Shin,Hyung‑Cheul; Won,Moo‑Ho

doi:10.3892/mmr.2014.2800

Increased immunoreactivity of c‑Fos in the spinal cord of the aged mouse and dog

Authors:
- Ji Hyeon Ahn
- Myoung Chul Shin
- Joon Ha Park
- In Hye Kim
- Jae‑Chul Lee
- Bing Chun Yan
- In Koo Hwang
- Seung Myung Moon
- Ji Yun Ahn
- Taek Geun Ohk
- Tae Hun Lee
- Jun Hwi Cho
- Hyung‑Cheul Shin
- Moo‑Ho Won
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Affiliations: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 200‑701, Republic of Korea, Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon 200‑701, Republic of Korea, Institute of Integrative Traditional and Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China, Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul 151‑742, Republic of Korea, Department of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong, Gyeonggi 445‑170, Republic of Korea, Department of Emergency Medicine, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon, Gangwon 200‑702, Republic of Korea, Department of Physiology, College of Medicine, Hallym University, Chuncheon, Gangwon 200‑702, Republic of Korea
Published online on: October 29, 2014 https://doi.org/10.3892/mmr.2014.2800
Pages: 1043-1048

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Abstract

Expression of c‑Fos in the spinal cord following nociceptive stimulation is considered to be a neurotoxic biomarker. In the present study, the immunoreactivity of c‑Fos in the spinal cord was compared between young adult (2‑3 years in dogs and 6 months in mice) and aged (10‑12 years in dogs and 24 months in mice) Beagle dogs and C57BL/6J mice. In addition, changes to neuronal distribution and damage to the spinal cord were also investigated. There were no significant differences in neuronal loss or degeneration of the spinal neurons observed in either the aged dogs or mice. Weak c‑Fos immunoreactivity was observed in the spinal neurons of the young adult animals; however, c‑Fos immunoreactivity was markedly increased in the nuclei of spinal neurons in the aged dogs and mice, as compared with that of the young adults. In conclusion, c‑Fos immunoreactivity was significantly increased without any accompanying neuronal loss in the aged spinal cord of mice and dogs, as compared with the spinal cords of the young adult animals.

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