Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells

Wei,Li; Yang,Xiaobo; Shi,Xiaohong; Chen,Yinghui

doi:10.3892/mmr.2015.3169

Pannexin‑1 silencing inhibits the proliferation of U87‑MG cells

Authors:
- Li Wei
- Xiaobo Yang
- Xiaohong Shi
- Yinghui Chen
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Affiliations: Department of Neurology, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China, Department of Endocrinology, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China
Published online on: January 8, 2015 https://doi.org/10.3892/mmr.2015.3169
Pages: 3487-3492

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Abstract

Pannexin‑1 (Panx‑1) is abundantly expressed in vertebrates and has been shown to assemble into high‑conductance single‑membrane channels, which are permeable to large molecules and regulate cellular function. However, the association between Panx‑1 and astrocyte proliferation is poorly understood. This study provides evidence for a difference in cell proliferation between wild‑type and Panx‑1‑knockdown cells. Proliferation of the U87‑MG malignant glioma cell line was reduced following transfection with Panx‑1‑short interfering RNA. In addition, treatment with the Panx‑1 activator, adenosine triphosphate, significantly reduced cell proliferation at 48 h in Panx‑1‑knockdown cells compared with wild type cells. In conclusion, on the basis of the present findings, Panx‑1 is likely to be important in the regulation of U87‑MG cell proliferation. This provides further support for the hypothesis that there is a correlation between Panx‑1 expression and U87‑MG cell proliferation.

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