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Ginsenoside Rg3 sensitizes human non‑small cell lung cancer cells to γ-radiation by targeting the nuclear factor-κB pathway

  • Authors:
    • Lei Wang
    • Xiankui Li
    • Yi‑Min Song
    • Bin Wang
    • Fu‑Rui Zhang
    • Rui Yang
    • Hua‑Qi Wang
    • Guo‑Jun Zhang

  • View Affiliations

  • Published online on: February 27, 2015     https://doi.org/10.3892/mmr.2015.3397
  • Pages: 609-614
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Abstract

At present, it is elusive how non‑small cell lung cancer (NSCLC) develops resistance to γ‑radiation; however, the transcription factor nuclear factor‑κB (NF‑κB) and NF‑κB‑regulated gene products have been proposed as mediators. Ginsenoside Rg3 is a steroidal saponin, which was isolated from Panax ginseng. Ginsenoside Rg3 possesses high pharmacological activity and has previously been shown to suppress NF‑κB activation in various types of tumor cell. Therefore, the present study aimed to determine whether Rg3 could suppress NF‑κB activation in NSCLC cells and sensitize NSCLC to γ‑radiation, using an NSCLC cell line and NSCLC xenograft. A clone formation assay and lung tumor xenograft experiment were used to assess the radiosensitizing effects of ginsenoside Rg3. NF‑κB/inhibitor of NF‑κB (IκB) modulation was ascertained using an electrophoretic mobility shift assay and western blot analysis. NF‑κB‑regulated gene products were monitored by western blot analysis. The present study demonstrated that ginsenoside Rg3 was able to sensitize A549 and H1299 lung carcinoma cells to γ‑radiation and significantly enhance the efficacy of radiation therapy in C57BL/6 mice bearing a Lewis lung carcinoma cell xenograft tumor. Furthermore, ginsenoside Rg3 suppressed NF‑κB activation, phosphorylation of IκB protein and expression of NF‑κB‑regulated gene products (cyclin D1, c‑myc, B‑cell lymphoma 2, cyclooxygenase‑2, matrix metalloproteinase‑9 and vascular endothelial growth factor), a number of which were induced by radiation therapy and mediate radioresistance. In conclusion, the results of the present study suggested that ginsenoside Rg3 may potentiate the antitumor effects of radiation therapy in NSCLC by suppressing NF‑κB activity and NF‑κB‑regulated gene products, leading to the inhibition of tumor progression.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Wang L, Li X, Song YM, Wang B, Zhang FR, Yang R, Wang HQ and Zhang GJ: Ginsenoside Rg3 sensitizes human non‑small cell lung cancer cells to γ-radiation by targeting the nuclear factor-κB pathway. Mol Med Rep 12: 609-614, 2015.
APA
Wang, L., Li, X., Song, Y., Wang, B., Zhang, F., Yang, R. ... Zhang, G. (2015). Ginsenoside Rg3 sensitizes human non‑small cell lung cancer cells to γ-radiation by targeting the nuclear factor-κB pathway. Molecular Medicine Reports, 12, 609-614. https://doi.org/10.3892/mmr.2015.3397
MLA
Wang, L., Li, X., Song, Y., Wang, B., Zhang, F., Yang, R., Wang, H., Zhang, G."Ginsenoside Rg3 sensitizes human non‑small cell lung cancer cells to γ-radiation by targeting the nuclear factor-κB pathway". Molecular Medicine Reports 12.1 (2015): 609-614.
Chicago
Wang, L., Li, X., Song, Y., Wang, B., Zhang, F., Yang, R., Wang, H., Zhang, G."Ginsenoside Rg3 sensitizes human non‑small cell lung cancer cells to γ-radiation by targeting the nuclear factor-κB pathway". Molecular Medicine Reports 12, no. 1 (2015): 609-614. https://doi.org/10.3892/mmr.2015.3397