Open Access

Overexpression of caudal type homeobox transcription factor 2 inhibits the growth of the MGC‑803 human gastric cancer cell line in vivo

  • Authors:
    • Weiyuan Wei
    • Lei Li
    • Xiaotong Wang
    • Linhai Yan
    • Wenlong Cao
    • Zexu Zhan
    • Xiaoshi Zhang
    • Han Yu
    • Yubo Xie
    • Qiang Xiao
  • View Affiliations

  • Published online on: March 4, 2015     https://doi.org/10.3892/mmr.2015.3413
  • Pages: 905-912
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Caudal type homeobox transcription factor 2 (CDX2) is important in intestinal cell fate specification and multiple lines of evidence have substantiated that CDX2 is important in carcinogenesis of the digestive tract. The CDX2 regulatory network is intricate and remains to be fully elucidated in gastric cancer. The aim of the present study was to examine the effects of CDX2 on the growth of the MGC‑803 human gastric cancer cell line in vivo, and to elucidate the mechanism involved. The effects of the overexpression of CDX2 in xenograft tumors of MGC‑803 cells was investigated in nude mice through the injection of CDX2 recombinant lentiviral vectors. The tumor size was measured using vernier callipers. The expression levels of CDX2, survivin, B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), cyclin D1, s‑phase kinase‑associated protein 2 (Skp2) and c‑Myc in the tumor cells were analyzed by western blotting and semi-quantitative reverse transcription polymerase chain reaction. The apoptotic rates were determined using a terminal deoxynucleotidyl transferase‑mediated dUTP‑biotin nick end labeling assay. The overexpression of CDX2 was observed in the group subjected to the injection of CDX2 recombinant lentiviral vectors. CDX2 had an inhibitory effect on the MGC‑803 human gastric cancer cell line and promoted tumor cell apoptosis in vivo. Furthermore, the overexpression of CDX2 upregulated the expression of Bax and downregulated the expression levels of survivin, Bcl‑2, cyclin D1, Skp2 and c‑Myc in the tumor tissues. These results indicated that CDX2 may serve as a tumor suppressor in gastric cancer, and inhibits gastric cancer cell growth by suppressing the nuclear factor‑κB signaling pathway.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Wei W, Li L, Wang X, Yan L, Cao W, Zhan Z, Zhang X, Yu H, Xie Y, Xiao Q, Xiao Q, et al: Overexpression of caudal type homeobox transcription factor 2 inhibits the growth of the MGC‑803 human gastric cancer cell line in vivo. Mol Med Rep 12: 905-912, 2015.
APA
Wei, W., Li, L., Wang, X., Yan, L., Cao, W., Zhan, Z. ... Xiao, Q. (2015). Overexpression of caudal type homeobox transcription factor 2 inhibits the growth of the MGC‑803 human gastric cancer cell line in vivo. Molecular Medicine Reports, 12, 905-912. https://doi.org/10.3892/mmr.2015.3413
MLA
Wei, W., Li, L., Wang, X., Yan, L., Cao, W., Zhan, Z., Zhang, X., Yu, H., Xie, Y., Xiao, Q."Overexpression of caudal type homeobox transcription factor 2 inhibits the growth of the MGC‑803 human gastric cancer cell line in vivo". Molecular Medicine Reports 12.1 (2015): 905-912.
Chicago
Wei, W., Li, L., Wang, X., Yan, L., Cao, W., Zhan, Z., Zhang, X., Yu, H., Xie, Y., Xiao, Q."Overexpression of caudal type homeobox transcription factor 2 inhibits the growth of the MGC‑803 human gastric cancer cell line in vivo". Molecular Medicine Reports 12, no. 1 (2015): 905-912. https://doi.org/10.3892/mmr.2015.3413