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All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation

  • Authors:
    • Geng‑Qiang Ling
    • Yi‑Jing Liu
    • Yi‑Quan Ke
    • Lei Chen
    • Xiao‑Dan Jiang
    • Chuan‑Lu Jiang
    • Wei Ye

  • View Affiliations

  • Published online on: March 6, 2015     https://doi.org/10.3892/mmr.2015.3449
  • Pages: 165-172

  • Copyright: © Ling et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The poor therapeutic effect of traditional antiangiogenic therapy on glioblastoma multiforme (GBM) may be attributed to vasculogenic mimicry (VM), which was previously reported to be promoted by cancer stem‑like cells (SLCs). All‑trans retinoic acid (ATRA), a potent reagent which drives differentiation, was reported to be able to eradicate cancer SLCs in certain malignancies. The aim of the present study was to investigate the effects of ATRA on the VM formation ability of U87 glioblastoma SLCs. The expression of cancer SLC markers CD133 and nestin was detected using immunocytochemistry in order to identify U87 SLCs. In addition, the differentiation of these SLCs was observed through detecting the expression of glial fibrillary acidic protein (GFAP), β‑tubulin III and galactosylceramidase (Galc) using immunofluorescent staining. The results showed that the expression levels of GFAP, β‑tubulin III and Galc were upregulated following treatment with ATRA in a dose‑dependent manner. Furthermore, ATRA significantly reduced the proliferation, invasiveness, tube formation and vascular endothelial growth factor (VEGF) secretion of U87 SLCs. In conclusion, the VM formation ability of SLCs was found to be negatively correlated with differentiation. These results therefore suggested that ATRA may serve as a promising novel agent for the treatment of GBM due to its role in reducing VM formation.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
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Spandidos Publications style
Ling GQ, Liu YJ, Ke YQ, Chen L, Jiang XD, Jiang CL and Ye W: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation. Mol Med Rep 12: 165-172, 2015.
APA
Ling, G., Liu, Y., Ke, Y., Chen, L., Jiang, X., Jiang, C., & Ye, W. (2015). All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation. Molecular Medicine Reports, 12, 165-172. https://doi.org/10.3892/mmr.2015.3449
MLA
Ling, G., Liu, Y., Ke, Y., Chen, L., Jiang, X., Jiang, C., Ye, W."All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation". Molecular Medicine Reports 12.1 (2015): 165-172.
Chicago
Ling, G., Liu, Y., Ke, Y., Chen, L., Jiang, X., Jiang, C., Ye, W."All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation". Molecular Medicine Reports 12, no. 1 (2015): 165-172. https://doi.org/10.3892/mmr.2015.3449