Chitosan oligosaccharides in combination with Agaricus blazei Murill extract reduces hepatoma formation in mice with severe combined immunodeficiency

Yeh,Ming‑Yang; Shang,Hung‑Sheng; Lu,Hsu‑Feng; Chou,Jason; Yeh,Chun; Chang,Jin‑Biou; Hung,Hsiao‑Fang; Kuo,Wan‑Lin; Wu,Lung‑Yuan; Chung,Jing‑Gung

doi:10.3892/mmr.2015.3454

Chitosan oligosaccharides in combination with Agaricus blazei Murill extract reduces hepatoma formation in mice with severe combined immunodeficiency

Authors:
- Ming‑Yang Yeh
- Hung‑Sheng Shang
- Hsu‑Feng Lu
- Jason Chou
- Chun Yeh
- Jin‑Biou Chang
- Hsiao‑Fang Hung
- Wan‑Lin Kuo
- Lung‑Yuan Wu
- Jing‑Gung Chung
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Affiliations: Department of Medical Education and Research, Cheng Hsin General Hospital, Taipei 112, Taiwan, R.O.C., Department of Pathology, National Defense Medical Center, Division of Clinical Pathology, Tri‑Service General Hospital, Taipei 112, Taiwan, R.O.C., Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei 112, Taiwan, R.O.C., Department of Anatomical Pathology, Cheng Hsin General Hospital, Taipei 112, Taiwan, R.O.C., Division of Gastroenterology, Cheng Hsin General Hospital, Taipei 112, Taiwan, R.O.C., Department of Medical Technology, Jen‑Teh Junior College of Medicine, Nursing and Management, Miaoli 356, Taiwan, R.O.C., Department of Biology, Ching Cheng High School, Changhua 500, Taiwan, R.O.C., School of Chinese Medicine for Post Baccalaureate, I‑Shou University, Kaohsiung 840, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan, R.O.C.
Published online on: March 9, 2015 https://doi.org/10.3892/mmr.2015.3454
Pages: 133-140
Copyright: © Yeh et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Chitosan and Agaricus blazei Murill (ABM) extracts possess antitumor activities. The aim of the present study was to investigate whether chitosan, ABM extract or the two in combination were effective against tumors in tumor‑bearing mice. The mice were subcutaneously injected with SK‑Hep 1 cells and were then were divided into the following six groups: Group 1, control group; group 2, chitosan 5 mg/kg/day; group 3, chitosan 20 mg/kg/day; group 4, ABM (246 mg/kg/day) and chitosan (5 mg/kg/day) combined; group 5, ABM (984 mg/kg/day) and chitosan (20 mg/kg/day) combined; and group 6, ABM (984 mg/kg/day). The mice were treated with the different concentrations of chitosan, ABM or combinations of the two for 6 weeks. The levels of glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and vascular endothelial growth factor (VEGF), and tissue histopathological features were examined in the surviving animals. Based on the results of the investigation, the treatments performed in groups 2, 3 and 4 were identified as being capable of reducing the weights of the tumors, however, group 4, which was treated with chitosan (5 mg/kg/day) in combination with ABM (246 mg/kg/day) was able to reduce the levels of GOT and VEGF. As a result, treatment with chitosan in combination with ABM may offer potential in cancer therapy and requires further investigation.

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