Effects of Schisandra chinensis Turcz. fruit on contact dermatitis induced by dinitrofluorobenzene in mice

Lee,Hee  Jung; Jo,Suzy; Ryu,Jeonghyun; Jeong,Han‑Sol; Lee,Guemsan; Ryu,Mi  Heon; Jung,Myeong  Ho; Kim,Hyungwoo; Kim,Byung  Joo

doi:10.3892/mmr.2015.3618

Effects of Schisandra chinensis Turcz. fruit on contact dermatitis induced by dinitrofluorobenzene in mice

Authors:
- Hee Jung Lee
- Suzy Jo
- Jeonghyun Ryu
- Han‑Sol Jeong
- Guemsan Lee
- Mi Heon Ryu
- Myeong Ho Jung
- Hyungwoo Kim
- Byung Joo Kim
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Affiliations: Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam-do 626‑870, Republic of Korea, Division of Pharmacology, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam-do 626‑870, Republic of Korea, Division of Applied Medicine, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam-do 626‑870, Republic of Korea, Department of Herbology, Wonkwang University College of Medicine, Iksan, North Jeolla 570‑749, Republic of Korea, Department of Oral Pathology, Pusan National University School of Dentistry, Yangsan, Gyeongsangnam-do 626‑870, Republic of Korea
Published online on: April 15, 2015 https://doi.org/10.3892/mmr.2015.3618
Pages: 2135-2139

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Abstract

Schisandra chinensis Turcz. fruit is widely used to treat skin diseases. The aim of this study was to determine the anti-inflammatory effects of the methanol extract of S. chinensis (MESC) on 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis (CD) in mice. The effects of MESC on ear thickness and weight, histopathological changes, immune cell filtration and cytokine production were investigated in DNFB-induced CD mice. Topical application of MESC effectively inhibited ear swelling (30 or 300 µg on the left ear, P<0.001; 30 µg on the right ear, P<0.001). MESC also inhibited hyperplasia, spongiosis (100 µg/ear, P<0.05 and 300 µg/ear, P<0.001, respectively) and immune cell infiltration (100 µg/ear, P<0.05; 300 µg/ear, P<0.001) induced by DNFB. In addition, MESC suppressed increases in tumor necrosis factor (TNF)‑α levels (100 or 300 µg/ear, P<0.05), interferon (INF)‑γ (30 µg/ear, P<0.05; 100 µg/ear, P<0.01; 300 µg/ear, P<0.001), interleukin (IL)‑6 (300 µg/ear, P<0.05) and monocyte chemoattractant protein (MCP)‑1 (30 µg/ear, P<0.05; 100 µg/ear, P<0.01; 300 µg/ear, P<0.001). These results suggest that the anti‑inflammatory effects of MESC are mediated by the reduced production of TNF‑α, IFN‑γ, IL‑6 and MCP‑1, and that MESC has potential use for the treatment of inflammatory skin diseases.

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