MicroRNA-9 suppresses the sensitivity of CNE2 cells to ultraviolet radiation

Zheng,Chao‑Pan; Han,Ling; Hou,Wei‑Jian; Tang,Jun; Wen,Yi‑Hui; Fu,Ran; Wang,Yue‑Jian; Wen,Wei‑Ping

doi:10.3892/mmr.2015.3622

MicroRNA-9 suppresses the sensitivity of CNE2 cells to ultraviolet radiation

Authors:
- Chao‑Pan Zheng
- Ling Han
- Wei‑Jian Hou
- Jun Tang
- Yi‑Hui Wen
- Ran Fu
- Yue‑Jian Wang
- Wei‑Ping Wen
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Affiliations: Department of Otorhinolaryngology, The First Affiliated Hospital and Otorhinolaryngology Institute, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Otolaryngology, First People's Hospital, Foshan, Guangdong 528000, P.R. China
Published online on: April 16, 2015 https://doi.org/10.3892/mmr.2015.3622
Pages: 2367-2373

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Abstract

MicroRNA (miR)-9 has been demonstrated to regulate the radiosensitivity of tumor cells. In the present study, the mechanism by which miR‑9 modulates the sensitivity of nasopharyngeal carcinoma (NPC) cells to ultraviolet (UV) radiation was investigated. The results demonstrated that exposure of NPC cells to UV light resulted in a significant increase in the expression of miR‑9, and that CNE2 cells overexpressing miR‑9 exhibited reduced levels of DNA damage and increased levels of total glutathione upon UV exposure. Accordingly, the inhibition of the expression of miR‑9 promoted UV‑induced DNA damage and apoptosis. Although miR‑9 inhibited the expression of E‑cadherin in the CNE2 cells and increased their resistance to UV radiation, the use of small interfering RNA to inhibit the expression of E‑cadherin was not sufficient to decrease the radiosensitivity of the NPC cells. These data demonstrated that miR‑9 did not modulate the sensitivity of the CNE2 cells to UV radiation through E‑cadherin, but suggested that miR‑9 regulated radiosensitivity through its effects on glutathione. These findings suggest that miR‑9 may be a potential target for modulating the radiosensitivity of NPC cells.

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