Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs

  • Authors:
    • Keran Song
    • Tao Gu
    • Feng Shuang
    • Jiaguang Tang
    • Dongfeng Ren
    • Jiang Qin
    • Shuxun Hou
  • View Affiliations

  • Published online on: June 8, 2015     https://doi.org/10.3892/mmr.2015.3895
  • Pages: 4664-4668
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Abstract

Patients with degenerative disc disease (DDD) experience serious clinical symptoms, including chronic low back pain. A series of therapies have been developed to treat DDD, including physical therapy and surgical treatment. However, the therapeutic effect of such treatments has remained insufficient. Recently, stem cell‑based therapy, in which stem cells are injected into the nucleus pulposus in degenerated intervertebral disc tissue, has appeared to be effective in the treatment of DDD. In the present study, the effect of adipose‑derived stem cells on degenerated nucleus pulposus cells was investigated using a co‑culture system to evaluate the biological activity of degenerated nucleus pulposus cells. Human degenerated nucleus pulposus tissue was obtained from surgical specimens and the adipose‑derived stem cells were derived from adipose tissue. The degenerated nucleus pulposus cells were cultured in a mono‑culture or in a co‑culture with adipose‑derived stem cells using 0.4‑µm Transwell inserts. The results indicated that adipose‑derived stem cells were able to stimulate matrix synthesis and the cell proliferation of degenerated nucleus pulposus cells, promoting the restoration of nucleus pulposus cells in the degenerated intervertebral disc.
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September-2015
Volume 12 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Song K, Gu T, Shuang F, Tang J, Ren D, Qin J and Hou S: Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs. Mol Med Rep 12: 4664-4668, 2015.
APA
Song, K., Gu, T., Shuang, F., Tang, J., Ren, D., Qin, J., & Hou, S. (2015). Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs. Molecular Medicine Reports, 12, 4664-4668. https://doi.org/10.3892/mmr.2015.3895
MLA
Song, K., Gu, T., Shuang, F., Tang, J., Ren, D., Qin, J., Hou, S."Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs". Molecular Medicine Reports 12.3 (2015): 4664-4668.
Chicago
Song, K., Gu, T., Shuang, F., Tang, J., Ren, D., Qin, J., Hou, S."Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs". Molecular Medicine Reports 12, no. 3 (2015): 4664-4668. https://doi.org/10.3892/mmr.2015.3895