Open Access

Overexpression of Indian hedgehog partially rescues short stature homeobox 2-overexpression‑associated congenital dysplasia of the temporomandibular joint in mice

  • Authors:
    • Xihai Li
    • Wenna Liang
    • Hongzhi Ye
    • Xiaping Weng
    • Fayuan Liu
    • Pingdong Lin
    • Xianxiang Liu
  • View Affiliations

  • Published online on: June 18, 2015     https://doi.org/10.3892/mmr.2015.3959
  • Pages: 4157-4164
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The role of short stature homeobox 2 (shox2) in the development and homeostasis of the temporomandibular joint (TMJ) has been well documented. Shox2 is known to be expressed in the progenitor cells and perichondrium of the developing condyle. A previous study by our group reported that overexpression of shox2 leads to congenital dysplasia of the TMJ via downregulation of the Indian hedgehog (Ihh) signaling pathway, which is essential for embryonic disc primordium formation and mandibular condylar growth. To determine whether overexpression of Ihh may rescue the overexpression of shox2 leading to congenital dysplasia of the TMJ, a mouse model in which Ihh and shox2 were overexpressed (Wnt1-Cre; pMes-stop shox2; pMes-stop Ihh mice) was utilized to assess the consequences of this overexpression on TMJ development during post-natal life. The results showed that the developmental process and expression levels of runt-related transcription factor 2 and sex determining region Y-box 9 in the TMJ of the Wnt1-Cre; pMes-stop shox2; pMes-stop Ihh mice were similar to those in wild‑type mice. Overexpression of Ihh rescued shox2 overexpression-associated reduction of extracellular matrix components. However, overexpression of Ihh did not inhibit the shox2 overexpression-associated increase of matrix metalloproteinases (MMPs) MMP9, MMP13 and apoptosis in the TMJ. These combinatory cellular and molecular defects appeared to account for the observed congenital dysplasia of TMJ, suggesting that overexpression of Ihh partially rescued shox2 overexpression‑associated congenital dysplasia of the TMJ in mice.
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September-2015
Volume 12 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li X, Liang W, Ye H, Weng X, Liu F, Lin P and Liu X: Overexpression of Indian hedgehog partially rescues short stature homeobox 2-overexpression‑associated congenital dysplasia of the temporomandibular joint in mice. Mol Med Rep 12: 4157-4164, 2015.
APA
Li, X., Liang, W., Ye, H., Weng, X., Liu, F., Lin, P., & Liu, X. (2015). Overexpression of Indian hedgehog partially rescues short stature homeobox 2-overexpression‑associated congenital dysplasia of the temporomandibular joint in mice. Molecular Medicine Reports, 12, 4157-4164. https://doi.org/10.3892/mmr.2015.3959
MLA
Li, X., Liang, W., Ye, H., Weng, X., Liu, F., Lin, P., Liu, X."Overexpression of Indian hedgehog partially rescues short stature homeobox 2-overexpression‑associated congenital dysplasia of the temporomandibular joint in mice". Molecular Medicine Reports 12.3 (2015): 4157-4164.
Chicago
Li, X., Liang, W., Ye, H., Weng, X., Liu, F., Lin, P., Liu, X."Overexpression of Indian hedgehog partially rescues short stature homeobox 2-overexpression‑associated congenital dysplasia of the temporomandibular joint in mice". Molecular Medicine Reports 12, no. 3 (2015): 4157-4164. https://doi.org/10.3892/mmr.2015.3959