Inhibitory effect of withaferin A on Helicobacter pylori‑induced IL‑8 production and NF‑κB activation in gastric epithelial cells

Kim,Green; Kim,Tae‑Hyoun; Kang,Min‑Jung; Choi,Jin‑A; Pack,Da‑Young; Lee,Ik‑Rae; Kim,Min‑Gyu; Han,Sang‑Seop; Kim,Bo‑Yeon; Oh,Sang‑Muk; Lee,Kyung‑Bok; Kim,Dong‑Jae; Park,Jong‑Hwan

doi:10.3892/mmr.2015.4602

Inhibitory effect of withaferin A on Helicobacter pylori‑induced IL‑8 production and NF‑κB activation in gastric epithelial cells

Authors:
- Green Kim
- Tae‑Hyoun Kim
- Min‑Jung Kang
- Jin‑A Choi
- Da‑Young Pack
- Ik‑Rae Lee
- Min‑Gyu Kim
- Sang‑Seop Han
- Bo‑Yeon Kim
- Sang‑Muk Oh
- Kyung‑Bok Lee
- Dong‑Jae Kim
- Jong‑Hwan Park
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Affiliations: Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302‑718, Republic of Korea, Laboratory Animal Medicine, College of Veterinary Medicine, Seoul University, Seoul 151‑742, Republic of Korea, Graduate School of Pre‑Clinical Laboratory Science, Konyang University, Daejeon 302‑718, Republic of Korea, World Class Institute, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑Gun, Choongbuk 363‑883, Republic of Korea
Published online on: November 23, 2015 https://doi.org/10.3892/mmr.2015.4602
Pages: 967-972

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Abstract

Withaferin A (WA), a withanolide purified from Withania somnifera, has been known to exert anti-inflammatory effects. The present study sought to determine the effects of WA on Helicobacter (H.) pylori-mediated inflammation in the AGS gastric epithelial cell line. Cellular production of interleukin (IL)-8 and vascular endothelial growth factor (VEGF) was measured by ELISA. Western blot analysis was performed to determine the activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) as well as hypoxia-inducible factor 1α stabilization. Bacterial growth was also examined by measuring the optical density. Pre-treatment or co-treatment with WA efficiently reduced IL-8 production by AGS cells in response to H. pylori infection. H. pylori-induced activation of NF-κB, but not MAPKs, was also inhibited by pre-treatment of WA in the cells. However, WA did not affect VEGF production and HIF-1α stabilization induced by H. pylori in AGS cells. In addition, WA did not influence the growth of H. pylori, suggesting that the anti-inflammatory effect of WA was not due to any bactericidal effect. These findings indicate that WA is a potential preventive or therapeutic agent for H. pylori-mediated gastric inflammation.

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