Open Access

Treatment with the herbal medicine, naoxintong improves the protective effect of high‑density lipoproteins on endothelial function in patients with type 2 diabetes

  • Authors:
    • Pu Lv
    • Xunliang Tong
    • Qing Peng
    • Yuanyuan Liu
    • Haiqiang Jin
    • Ran Liu
    • Wei Sun
    • Bing Pan
    • Lemin Zheng
    • Yining Huang
  • View Affiliations

  • Published online on: January 18, 2016     https://doi.org/10.3892/mmr.2016.4792
  • Pages: 2007-2016
  • Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The protective effect of high‑density lipoprotein (HDL) on endothelial function is impaired in patients with type 2 diabetes mellitus (T2DM), which may result in atherosclerotic complications. Naoxintong (NXT) is a compound preparation that includes Radix Astragali, Angelicae sinensis, Radix Paeoniae Rubra and Ligusticum wallichii. It is widely administered in China to prevent atherosclerotic complications. In the present study, NXT was administered to 69 patients with T2DM. HDLs were isolated from patient blood samples prior to and following the intervention. In vitro endothelial functions of HDL, including proliferation, migration, angiogenesis, and anti‑apoptosis were investigated by bromodeoxyuridine, wound healing, Transwell and Matrigel tube formation assays on human umbilical vein endothelial cells (HUVECs). The results from the present study demonstrated that HUVECs treated with HDL isolated from diabetic patients following NXT therapy exhibited increased proliferative effects (10‑27%; P<0.05), and improved migration ability (15‑35%; P<0.05), anti‑apoptotic function (23‑34%; P<0.05) and angiogenesis (30‑54%; P<0.001). Furthermore, the phosphorylation levels of Akt (26‑36%; P<0.01) and extracellular signal‑regulated kinase (16‑80%; P<0.01) were increased following NXT therapy. The present in vitro study demonstrates that the protective effect of HDL on endothelial function is markedly impaired in diabetic patients who tend to develop atherosclerosis, and the impaired function may be partly abrogated by NXT.
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March-2016
Volume 13 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Lv P, Tong X, Peng Q, Liu Y, Jin H, Liu R, Sun W, Pan B, Zheng L, Huang Y, Huang Y, et al: Treatment with the herbal medicine, naoxintong improves the protective effect of high‑density lipoproteins on endothelial function in patients with type 2 diabetes. Mol Med Rep 13: 2007-2016, 2016.
APA
Lv, P., Tong, X., Peng, Q., Liu, Y., Jin, H., Liu, R. ... Huang, Y. (2016). Treatment with the herbal medicine, naoxintong improves the protective effect of high‑density lipoproteins on endothelial function in patients with type 2 diabetes. Molecular Medicine Reports, 13, 2007-2016. https://doi.org/10.3892/mmr.2016.4792
MLA
Lv, P., Tong, X., Peng, Q., Liu, Y., Jin, H., Liu, R., Sun, W., Pan, B., Zheng, L., Huang, Y."Treatment with the herbal medicine, naoxintong improves the protective effect of high‑density lipoproteins on endothelial function in patients with type 2 diabetes". Molecular Medicine Reports 13.3 (2016): 2007-2016.
Chicago
Lv, P., Tong, X., Peng, Q., Liu, Y., Jin, H., Liu, R., Sun, W., Pan, B., Zheng, L., Huang, Y."Treatment with the herbal medicine, naoxintong improves the protective effect of high‑density lipoproteins on endothelial function in patients with type 2 diabetes". Molecular Medicine Reports 13, no. 3 (2016): 2007-2016. https://doi.org/10.3892/mmr.2016.4792