Open Access

RNA sequencing reveals retinal transcriptome changes in STZ‑induced diabetic rats

  • Authors:
    • Yuan‑Jie Liu
    • Zhi‑Yun Lian
    • Geng Liu
    • Hong‑Ying Zhou
    • Hui‑Jun Yang
  • View Affiliations

  • Published online on: January 18, 2016     https://doi.org/10.3892/mmr.2016.4793
  • Pages: 2101-2109
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate changes in retinal gene expression in streptozotocin (STZ)‑induced diabetic rats using next‑generation sequencing, utilize transcriptome signatures to investigate the molecular mechanisms of diabetic retinopathy (DR), and identify novel strategies for the treatment of DR. Diabetes was chemically induced in 10‑week‑old male Sprague‑Dawley rats using STZ. Flash‑electroretinography (F‑ERG) was performed to evaluate the visual function of the rats. The retinas of the rats were removed to perform high throughput RNA sequence (RNA‑seq) analysis. The a‑wave, b‑wave, oscillatory potential 1 (OP1), OP2 and ∑OP amplitudes were significantly reduced in the diabetic group, compared with those of the control group (P<0.05). Furthermore, the implicit b‑wave duration 16 weeks post‑STZ induction were significantly longer in the diabetic rats, compared with the control rats (P<0.001). A total of 868 genes were identified, of which 565 were upregulated and 303 were downregulated. Among the differentially expressed genes (DEGs), 94 apoptotic genes and apoptosis regulatory genes, and 19 inflammatory genes were detected. The results of the KEGG pathway significant enrichment analysis revealed enrichment in cell adhesion molecules, complement and coagulation cascades, and antigen processing and presentation. Diabetes alters several transcripts in the retina, and RNA‑seq provides novel insights into the molecular mechanisms underlying DR.
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March-2016
Volume 13 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Liu YJ, Lian ZY, Liu G, Zhou HY and Yang HJ: RNA sequencing reveals retinal transcriptome changes in STZ‑induced diabetic rats. Mol Med Rep 13: 2101-2109, 2016
APA
Liu, Y., Lian, Z., Liu, G., Zhou, H., & Yang, H. (2016). RNA sequencing reveals retinal transcriptome changes in STZ‑induced diabetic rats. Molecular Medicine Reports, 13, 2101-2109. https://doi.org/10.3892/mmr.2016.4793
MLA
Liu, Y., Lian, Z., Liu, G., Zhou, H., Yang, H."RNA sequencing reveals retinal transcriptome changes in STZ‑induced diabetic rats". Molecular Medicine Reports 13.3 (2016): 2101-2109.
Chicago
Liu, Y., Lian, Z., Liu, G., Zhou, H., Yang, H."RNA sequencing reveals retinal transcriptome changes in STZ‑induced diabetic rats". Molecular Medicine Reports 13, no. 3 (2016): 2101-2109. https://doi.org/10.3892/mmr.2016.4793