Resveratrol attenuates inflammatory hyperalgesia by inhibiting glial activation in mice spinal cords

  • Authors:
    • Lin‑Lin Wang
    • Dong‑Ling Shi
    • Hui‑Yao Gu
    • Ming‑Zhi Zheng
    • Jue Hu
    • Xing‑Hui Song
    • Yue‑Liang Shen
    • Ying‑Ying Chen
  • View Affiliations

  • Published online on: March 21, 2016     https://doi.org/10.3892/mmr.2016.5027
  • Pages: 4051-4057
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Abstract

The present study aimed to investigate the effect of resveratrol on inflammatory pain. Mice were injected intraperitoneally with lipopolysaccharide (LPS) for 5 consecutive days to induce subacute systemic inflammation. Acetic acid‑induced writhing tests and tail‑flick tests were performed following the final LPS injection. Glial fibrillary acidic protein (GFAP; an astrocyte‑specific activation marker), ionized calcium binding adapter molecule 1 (Iba‑1; a microglia‑specific activation marker) and sirtuin 1 (SIRT1) protein expression levels were detected using immunohistochemistry analysis or western blotting. Following administration of LPS for 5 days, the number of writhes increased and the tail‑flick latency decreased. Resveratrol (10 or 20 mg/kg) partly inhibited LPS‑induced hyperalgesia and prevented the increase in tumor necrosis factor‑α and interleukin 6 levels induced by LPS. LPS injection reduced the SIRT1 protein expression and increased the number of GFAP‑positive and Iba‑1‑positive cells in the spinal cord. Resveratrol increased the SIRT1 protein expression levels and decreased the number of GFAP‑positive and Iba‑1‑positive cells in LPS‑treated mice. The protective effect of resveratrol was partly blocked by a selective SIRT1 inhibitor, EX‑257. Results from the present study suggest that subacute treatment with LPS induced the activation of glial cells and hyperalgesia. Resveratrol was demonstrated to inhibit the activation of glial cells and attenuate inflammatory hyperalgesia in a SIRT1‑dependent manner.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang LL, Shi DL, Gu HY, Zheng MZ, Hu J, Song XH, Shen YL and Chen YY: Resveratrol attenuates inflammatory hyperalgesia by inhibiting glial activation in mice spinal cords. Mol Med Rep 13: 4051-4057, 2016.
APA
Wang, L., Shi, D., Gu, H., Zheng, M., Hu, J., Song, X. ... Chen, Y. (2016). Resveratrol attenuates inflammatory hyperalgesia by inhibiting glial activation in mice spinal cords. Molecular Medicine Reports, 13, 4051-4057. https://doi.org/10.3892/mmr.2016.5027
MLA
Wang, L., Shi, D., Gu, H., Zheng, M., Hu, J., Song, X., Shen, Y., Chen, Y."Resveratrol attenuates inflammatory hyperalgesia by inhibiting glial activation in mice spinal cords". Molecular Medicine Reports 13.5 (2016): 4051-4057.
Chicago
Wang, L., Shi, D., Gu, H., Zheng, M., Hu, J., Song, X., Shen, Y., Chen, Y."Resveratrol attenuates inflammatory hyperalgesia by inhibiting glial activation in mice spinal cords". Molecular Medicine Reports 13, no. 5 (2016): 4051-4057. https://doi.org/10.3892/mmr.2016.5027