Knockdown of RHOC by shRNA suppresses invasion and migration of cholangiocellular carcinoma cells via inhibition of MMP2, MMP3, MMP9 and epithelial-mesenchymal transition

Yang,Haixia; Liang,Jun; Zhou,Jiupeng; Mi,Jianqiang; Ma,Ke; Fan,Yangwei; Ning,Jing; Wang,Chuying; Wei,Xin; Li,Enxiao

doi:10.3892/mmr.2016.5170

Knockdown of RHOC by shRNA suppresses invasion and migration of cholangiocellular carcinoma cells via inhibition of MMP2, MMP3, MMP9 and epithelial-mesenchymal transition

Authors:
- Haixia Yang
- Jun Liang
- Jiupeng Zhou
- Jianqiang Mi
- Ke Ma
- Yangwei Fan
- Jing Ning
- Chuying Wang
- Xin Wei
- Enxiao Li
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Affiliations: Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Medical Oncology, Xi'an Chest Hospital of Shaanxi, Xi'an, Shaanxi 710061, P.R. China, Department of Pathology, First Affiliated Hospital of Henan Science and Technology University, Luoyang, Henan 471003, P.R. China, Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710062, P.R. China
Published online on: April 22, 2016 https://doi.org/10.3892/mmr.2016.5170
Pages: 5255-5261

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Abstract

Ras homolog family member C (RHOC) is important during the progression of several types of cancer, including prostate, breast and hepatocellular carcinoma. However, the function of RHOC in cholangiocellular carcinoma (CCC), a highly recurrent and metastatic carcinoma with poor prognosis, remains unclear. The aim of the present study was to investigate the involvement of RHOC in CCC tumor progression. RHOC expression levels were examined in CCC tissues and cells, and adjacent nontumorous bile duct tissues. The effects and molecular mechanisms of RHOC expression on cell migration and invasion were also investigated. The current study demonstrated that RHOC protein was frequently overexpressed in human CCC specimens and CCC cell lines. Downregulation of RHOC inhibited CCC cell invasion and migration partially via inhibition of matrix metalloproteinase 2, 3 and 9 expression. RHOC also modulated the expression of several epithelial-mesenchymal transition (EMT)-associated proteins, including E‑cadherin, vimentin, Slug and Snail, to promote to EMT progression. The present results demonstrated that RHOC is important for the invasion and migration of CCC through simultaneous regulation of MMPs and EMT‑associated protein, suggesting that RHOC is a potential molecular target for CCC treatment.

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