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Gene expression profiling of NB4 cells following knockdown of nucleostemin using DNA microarrays
- Authors:
- Published online on: May 5, 2016 https://doi.org/10.3892/mmr.2016.5213
- Pages: 175-183
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Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
In 2002, nucleostemin (NS) was detected in the nucleoli of early pluripotent cells, and was found to be associated with cell proliferation; it is also crucial for supporting the undifferentiated properties of self-renewal certain types of stem cell (1,2) as well as germ cell tumors (3). Furthermore, NS is abundantly expressed in numerous tumor types, including prostate cancer (4), esophageal cancer (5), breast carcinoma (6) and gastric adenocarcinoma (7). Elevated NS expression is associated with poor prognosis of patients with various types of cancer (8,9). Furthermore, knockdown of NS was shown to inhibit cell proliferation as well as induce cell cycle arrest and apoptosis (6,10–13). NS is therefore a potential biomarker for tumor diagnosis and prognosis (7,14).
Originally, NS was reported to combine with p53 to inhibit its function as a tumor suppressor (1). However, subsequent studies have revealed an additional p53-independent role for NS (15–17). It has been reported that NS regulates the cell cycle by modulating the stability of ARF tumor suppressor (18). However, to date, the detailed mechanism of the p53-independent NS pathway has remained elusive.
Patients with wild-type p53 tumors generally have a better prognosis than those p53-null or p53-mutant tumors; however, the latter represent the majority of tumors, which is a reason for drug-resistance and poor therapeutic effects (19). Thus, it is urgent to explore effective treatment targets for tumors which are p53-null or p53-mutant. A previous study by our group has performed gene expression profiling of the p53-null HL-60 leukemia cell line following NS knockdown (20). In order to further explore the p53-independent NS pathway, the present study subjected the p53-mutant NB4 leukemia cell line to DNA microarray analysis and gene expression profiling following NS knockdown. The present study shed light on the mechanisms of the p53-independent NS pathway in NB4 cells and provided a foundation for the discovery of promising targets for the treatment of p53-mutant leukemia.
Materials and methods
Cell culture
NB4 cells (GeneChem Co., Ltd., Shanghai, China) were maintained in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C in a humidified atmosphere containing 5% CO2. The medium was replaced every two days.
Lentiviral NS-small interfering (si)RNA vector construction, packaging and transfection
The siRNA target sequence (5′-CAAGTATTGAAGTAGTAAA-3′) for the NS gene (Genbank ID, NM_004196) was designed by GeneChem Co., Ltd. The two different single-stranded DNA oligonucleotides (Table I, designed by GeneChem Co., Ltd.) were matched to generate the NS-siRNA constructs by being dissolved in buffer, placed in 90°C water bath for 15 min and then naturally cooled to room temperature. Then the NS-siRNA constructs were inserted into the green fluorescent protein-labelled lentiviral expression vector GV248 (Genechem Co., Ltd.) to form the recombinant vector named NS-RNAi-GV248 vector. Next, the recombinant NS-RNAi-GV248 vectors were transformed into competent Escherichia coli cells (GeneChem Co., Ltd.) and then verified by DNA sequencing using 3730XL Genetic analyzer (Applied Biosystems, Thermo Fisher Scientific, Inc.). Subsequently, the recombinant vectors NS-RNAi-GV248, the packaging vectors pHelper 1.0 and pHelper 2.0 (GeneChem Co., Ltd.) were co-transfected into 293T cells (GeneChem Co., Ltd.). The packaged vectors were collected from the supernatants of the cell culture medium at 48 h after transfection. Then the Lentiviral Purification kit (GeneChem Co., Ltd.) was used to concentrate and purify the packaged recombinant lentiviral vectors according to the manufacturer's protocol.
For lentiviral transfection, 1×106 NB4 cells in the logarithmic growth phase were seeded into six-well plates with 2 ml fresh medium well. According to the titer of NB4 cells (4×108 ml) and multiplicity of infection (MOI, 30), 80 µl of lentivirus were added to each well. A negative control group treated with lentiviral vectors containing negative control sequence: Sense 5′-UUCUCCGAACGUGUCACGUTT-3′; antisense 5′-ACGUGACACGUUCGGAGAATT-3′ (GeneChem Co., Ltd.) and a blank control group without any lentivirus treatment was also established. At 16 h after infection, the culture medium was replaced with pure medium and at 72 h after infection, the cells were observed under a fluorescence microscope (Eclipse TS100; Nikon Corporation, Tokyo, Japan) to evaluate the transfection efficiency.
RNA extraction and reverse-transcription quantitative polymerase chain reaction analysis (RT-qPCR)
At 96 h after transfection, 5–10×106 cells per group were collected and total RNA was isolated using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. The total extracted RNA was used to synthesize cDNA by reverse transcription reaction using the PrimeScript RT Reagent kit with gDNA Eraser (Takara Bio, Inc., Otsu, Japan). PCR amplification of NS, GAPDH, CCND2, CHOP, MTIE, MTIF and MAPK9 was performed in an ABI7500 quantitative real-time PCR instrument (Thermo Fisher Scientific, Inc.) using the SYBR Premix Ex Taq II (Tli RNaseH Plus) kit (Takara Bio, Inc.) and the corresponding primers as listed in Table II were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). The following thermocycling conditions were used: 95°C for 30 sec; 95°C for 5 sec, 60°C for 34 sec, 40 cycles; 95°C for 15 sec, 60°C for 1 min, 95°C for 15 sec. The PCR products were quantified using the 2−ΔΔCq method (21)
Microarray hybridization and data processing
For each treatment group of cells, the total extracted RNA was purified using an RNase Mini kit (cat. no. 74104; Qiagen, Hilden, Germany) and reversely transcribed into cDNA. Cy3-labelled cRNA was synthesized from cDNA using the Quick Amp Labeling kit, One-Color (cat. no. 5190-0442; Agilent Technologies, Inc., Santa Clara, CA, USA). The purified labelled cRNA was then subjected to hybirdization using Agilent 4×44K Human Whole-Genome 60-mer oligonucleotide microarrays with utilization of the Agilent Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.) following the manufacturer's instructions.
An Agilent DNA microarray scanner (cat. no. G2565BA; Agilent Technologies, Inc.) was used to scan the microarrays, with the parameters set as follows: Green photomultiplier tube, external data representation (XDR) Hi 100% and XDR Lo 10%; scan resolution, 5 µm. Next, the acquired microarray images were analyzed using Feature Extraction v 11.01.1 software (Agilent Technologies, Inc.), and the resulting text files extracted from it were further analyzed by GeneSpring GX v 12.0 software (Agilent Technologies, Inc.). The data were normalized through logarithmic transformation. Genes with low expression were removed genes detected in all samples were selected for further data analysis. Only genes with a fold change ≥2 or ≤0.5 were considered as differentially expressed between the experimental and the negative control groups. Finally, the differentially expressed genes were subjected to functional analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database (http://www.genome.jp/kegg).
Statistical analysis
Each assay was performed in triplicate, and values are expressed as the mean ± standard deviation. SPSS 17.0 statistical software (SPSS, Inc., Chicago, IL, USA) was used for analysis. The means of two groups were compared using Student's t-test. Fisher's exact test was applied to assess the significance in the pathway analysis. P<0.05 was considered to indicate a statistically significant difference between values.
Results
Transfection of NB4 cells with NS-siRNA lentiviral vectors
Observation under the inverted fluorescence microscope revealed that the transfection efficiency of the lentiviral vectors was >80% (Fig. 1). The NS mRNA expression levels in the experimental group were decreased by ~81% compared with the blank control and the negative control group (P<0.05), as revealed by RT-qPCR. In order to minimize the off-target effect of NS-siRNA lentiviral vectors, cells from the negative control-transfected group were then subjected to DNA micro-array analysis alongside the experimental group.
DNA microarray data analysis
With the filter cutoff set at a 2.0-fold change in the microarray data analysis, a total of 1,953 differentially expressed genes were identified in NB4 cells following knockdown of NS. Of these genes, 943 were upregulated and 1,010 genes were downregulated.
Confirmation of the microarray data by RT-qPCR analysis
To further confirm the reliability of the microarray data, four significantly differentially expressed genes, CCND2, CHOP, MT1E, MT1F and MAPK9, were selected for RT-qPCR analysis. The results of the RT-qPCR analysis were in general agreement with the microarray data, as they showed the same trends (Fig. 2).
Pathway analysis
The differentially expressed genes were subjected to pathway analysis based on the KEGG database. The significant pathways containing an accumulation of upregulated or downregulated genes are listed in Tables III and IV, respectively.
Discussion
NS is a protein required for the maintenance of stem cells, the early embryonal development and proliferation of tumor cells (1,4,5). While NS was initially indicated to act via combining with p53 (1), increasing evidence suggested the existence of an additional p53-independent NS pathway (15–17,22–24). However, the underlying mechanisms of the function of NS in p53-inactivated tumor cells have largely remained elusive. A previous study by our group reported that inhibition of the JAK/STAT, the PI3K/AKT and the RAS/RAF/MEK/ERK1/2 pathways, as well as the activation of the p38MAPK and JNK pathways may participate in the induction of apoptosis following NS knockdown in p53-null HL-60 cells (20).
The present study further explored the mechanisms of the function of the p53-independent function of NS by using the p53-mutant NB4 leukemia cell line. Gene expression profiling indicated that a large number of genes were aberrantly expressed in NB4 cells following knockdown of NS.
Subsequent pathway analysis of upregulated genes revealed that protein processing in the endoplasmic reticulum (ER) was the most significant KEGG pathway. Under normal physiological conditions, the ER only transports correctly folded proteins to the Golgi-apparatus, while misfolded proteins are extracted for ubiquitin-dependent ER-associated degradation (ERAD) (25). However, insufficient degradation leads to ER stress due to accumulation of misfolded proteins, and the unfolded protein response (UPR) is then activated to maintain the homeostasis of the ER (26). When misfolded protein stress exceeds the tolerance threshold of the ER, and the UPR is insufficient for the maintenance of homeostasis, cell apoptosis is activated, which is referred to as ER stress-induced apoptosis (27,28). PERK, IRE1 and ATF6 are three main sensor proteins located on the ER membrane, which are activated as part of the UPR by dissociation from GRP78 and relieve the stress through a series of pathways. As illustrated in Fig. 3, a number of upregulated genes were associated with the ERAD process, which indicated that ER stress may have increased in NB4 cells after knockdown of NS. Furthermore, prolonged ER stress is able to induce apoptosis (29). However, further studies are required to determine whether downregulation of NS may directly activate apoptosis in NB4 cells. CHOP and JNK are key mediators during ER stress-induced apoptosis (30). In the present study, PERK, CHOP and JNK (MAPK9) were all upregulated. Therefore, it is indicated that the p53-independent NS pathway may also be associated with increases in ER stress and an imbalance of ER homeostasis.
In addition, several of the upregulated genes were enriched in the MAPK signaling pathway (Fig. 4), which was a similar finding to previous observations in HL-60 cells (20). MAPK pathways mainly consist of the JNK, the RAS/RAF/MEK/ERK2 and the p38MAPK pathways, while activation of JNK and p38MAPK pathways is known to induce cell apoptosis (31). In contrast to the findings in HL-60 cells, only JNK(MAPK9) was upregulated in NB4 cells following NS knockdown. Therefore, activation of the JNK pathway was another effect of NS knockdown in NB4 cells, indicating that NS may exert its effects via the NS pathway.
Analysis of downregulated genes showed that mineral absorption was most significant pathway. The key genes in this pathway were metallothioneins (MTs). MT genes are a family of cysteine-rich proteins, which are closely linked and mainly comprise 11 MT-1 genes (including MT-1A, -B, -E - L and -X) and one gene for other MT isoforms (MT-2A, MT-3 and MT-4) (32). MT-1 and MT-2 isoforms are widely expressed in numerous cell types. MT-3 is associated with neuronal cells; it is also called neuronal growth-inhibitory factor and inhibits the outgrowth of neuronal cells (33). MT-4 is primarily expressed on certain squamous epithelial cells (34). The differentially expressed MT genes in the present study were mainly MT-1 genes, including MT-1A, MT-1B, MT-1E, MT-1F, MT-1H, MT-1L and MT-1X. MTs participate in zinc and copper homeostasis, protection against heavy metal toxicity and oxidative damage. As zinc deficiency is associated with oxidative stress (35), downregulation of MTs may disturb the homeostasis of zinc and copper, which increases oxidative stress in cells. Furthermore, as MTs are rich in sulfhydryl and have a high anti-oxidant capacity, they are able to effectively eliminate superoxide and hydroxyl radicals (36). Therefore, downregulation of MT expression may result in oxidative stress due to the absence of anti-oxidants, which may be an underlying mechanism for the imbalance of ER homeostasis indicated by the present study.
In addition, MTs are closely linked with malignant tumors. Metal-regulatory transcription factor-1 (MTF-1) is the only known mediator of the metal responsiveness of MTs and is able to regulate the expression of MTs (32,37,38). MTF-1 is elevated in numerous tumor types, including lung, breast, and cervical carcinoma-derived cell lines (39), which supports the link between MTs and tumors. MTs have been shown to be distinctly elevated in rapidly growing tissues such as the neonatal liver, suggesting that MTs are important in cell proliferation (40,41). Furthermore, increased expression of MTs is associated with drug resistance (42,43), anti-apoptotic capacity (44,45), breast cancer prognosis (46) and differentiation of thyroid tumor cells (47). Thus, the DNA microarray data analysis indicated that following a knockdown of NS in NB4 cells, diminished proliferation and low tumorigenic capacity may occur due to downregulation of MTs. This requires further verification in future studies.
In the present study, a further significant pathway with accumulation of downregulated genes was the peroxisome pathway. Inhibition of the peroxisome pathway reduces the synthesis of peroxisome, which is an intracellular organelle located in the cytoplasm and contains an abundance of enzymes, including catalase, oxidase and peroxidase. Therefore, decreased synthesis of peroxisome reduces these enzymes, which may influence the redox state of the cells or the ER, causing imbalance of ER homeostasis.
In addition, pathway analysis of downregulated genes showed that the pathways involved were mostly associated with biosubstance synthesis and metabolism, including sulfur metabolism, RNA transport, N-glycan biosynthesis, fructose and mannose metabolism, ribosome biogenesis in eukaryotes, glycosaminoglycan biosynthesis and citrate cycle. This indicated that the biosynthesis and metabolism of NB4 cells was reduced following knockdown of NS expression (48,49).
In conclusion, gene expression profiling analysis of NB4 cells following knockdown of NS revealed a large number of differentially expressed genes. Pathway analysis indicated that ER stress may increase in NB4 cells after NS inhibition, which may cause an imbalance of ER homeostasis; furthermore, the JNK pathway was activated. In addition, biosubstance synthesis and metabolism in NB4 cells was reduced following NS knockdown. The present study provided insight into the underlying mechanism of the p53-independent NS signaling pathway, which may be utilized for the development of novel treatments for p35-null/mutated cancer types.
Acknowledgments
The present study was funded by the National Natural Science Foundation of China (no. 81271911) and the Key Projects of Medical Science and Technology of Henan Province (no. 201002006).
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