Comparison of catalase immunoreactivity in the hippocampus between young, adult and aged mice and rats

Ahn,Ji  Hyeon; Chen,Bai  Hui; Shin,Bich‑Na; Lee,Tae‑Kyeong; Cho,Jeong  Hwi; Kim,In  Hye; Park,Joon  Ha; Lee,Jae‑Chul; Tae,Hyun‑Jin; Lee,Choong‑Hyun; Won,Moo‑Ho; Lee,Yun  Lyul; Choi,Soo  Young; Hong,Seongkweon

doi:10.3892/mmr.2016.5300

Comparison of catalase immunoreactivity in the hippocampus between young, adult and aged mice and rats

Authors:
- Ji Hyeon Ahn
- Bai Hui Chen
- Bich‑Na Shin
- Tae‑Kyeong Lee
- Jeong Hwi Cho
- In Hye Kim
- Joon Ha Park
- Jae‑Chul Lee
- Hyun‑Jin Tae
- Choong‑Hyun Lee
- Moo‑Ho Won
- Yun Lyul Lee
- Soo Young Choi
- Seongkweon Hong
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Affiliations: Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon-do 24252, Republic of Korea, Department of Physiology, College of Medicine, Hallym University, Chuncheon, Gangwon-do 24252, Republic of Korea, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea, Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam-do 31116, Republic of Korea, Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea
Published online on: May 18, 2016 https://doi.org/10.3892/mmr.2016.5300
Pages: 851-856
Copyright: © Ahn et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Catalase (CAT) is an important antioxidant enzyme and is crucial in modulating synaptic plasticity in the brain. In this study, CAT expression as well as neuronal distribution was compared in the hippocampus among young, adult and aged mice and rats. Male ICR mice and Sprague Dawley rats were used at postnatal month (PM) 1, PM 6 and PM 24 as the young, adult and aged groups, respectively (n=14/group). CAT expression was examined by immunohistochemistry and western blot analysis. In addition, neuronal distribution was examined by NeuN immunohistochemistry. In the present study, the mean number of NeuN‑immunoreactive neurons was marginally decreased in mouse and rat hippocampi during aging, although this change was not identified to be significantly different. However, CAT immunoreactivity was significantly increased in pyramidal and granule neurons in the adult mouse and rat hippocampi and was significantly decreased in the aged mouse and rat hippocampi compared with that in the young animals. CAT protein levels in the hippocampus were also lowest in the aged mouse and rat hippocampus. These results indicate that CAT expression is significantly decreased in the hippocampi of aged animals and decreased CAT expression may be closely associated with aging.

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