Open Access

Effects of imatinib and nilotinib on the whole transcriptome of cultured murine osteoblasts

  • Authors:
    • Gyöngyi Kirschner
    • Bernadett Balla
    • Péter Horváth
    • Andrea Kövesdi
    • Gergely Lakatos
    • István Takács
    • Zsolt Nagy
    • Bálint Tóbiás
    • Kristóf Árvai
    • János Pál Kósa
    • Péter Lakatos
  • View Affiliations

  • Published online on: June 30, 2016     https://doi.org/10.3892/mmr.2016.5459
  • Pages: 2025-2037
  • Copyright: © Kirschner et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Numerous clinical observations have confirmed that breakpoint cluster region-abelson fusion oncoprotein tyrosine kinase inhibitors used in leukemia treatment alter bone physiology in a complex manner. The aim of the present study was to analyze the whole transcriptome of cultured murine osteoblasts and determine the changes following treatment with imatinib and nilotinib using Sequencing by Oligonucleotide Ligation and Detection next generation RNA sequencing. This study also aimed to identify candidate signaling pathways and network regulators by multivariate Ingenuity Pathway Analysis. Based on the right-tailed Fisher's exact test, significantly altered pathways including upstream regulators were defined for each drug. The correlation between these pathways and bone metabolism was also examined. The preliminary results suggest the two drugs have different mechanisms of action on osteoblasts, and imatinib was shown to have a greater effect on gene expression. Data also indicated the potential role of a number of genes and signaling cascades that may contribute to identifying novel targets for the treatment of metabolic bone diseases.
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September-2016
Volume 14 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kirschner G, Balla B, Horváth P, Kövesdi A, Lakatos G, Takács I, Nagy Z, Tóbiás B, Árvai K, Kósa JP, Kósa JP, et al: Effects of imatinib and nilotinib on the whole transcriptome of cultured murine osteoblasts. Mol Med Rep 14: 2025-2037, 2016.
APA
Kirschner, G., Balla, B., Horváth, P., Kövesdi, A., Lakatos, G., Takács, I. ... Lakatos, P. (2016). Effects of imatinib and nilotinib on the whole transcriptome of cultured murine osteoblasts. Molecular Medicine Reports, 14, 2025-2037. https://doi.org/10.3892/mmr.2016.5459
MLA
Kirschner, G., Balla, B., Horváth, P., Kövesdi, A., Lakatos, G., Takács, I., Nagy, Z., Tóbiás, B., Árvai, K., Kósa, J. P., Lakatos, P."Effects of imatinib and nilotinib on the whole transcriptome of cultured murine osteoblasts". Molecular Medicine Reports 14.3 (2016): 2025-2037.
Chicago
Kirschner, G., Balla, B., Horváth, P., Kövesdi, A., Lakatos, G., Takács, I., Nagy, Z., Tóbiás, B., Árvai, K., Kósa, J. P., Lakatos, P."Effects of imatinib and nilotinib on the whole transcriptome of cultured murine osteoblasts". Molecular Medicine Reports 14, no. 3 (2016): 2025-2037. https://doi.org/10.3892/mmr.2016.5459