search button
Open Access

Directed differentiation of postnatal hippocampal neural stem cells generates nuclear receptor related‑1 protein‑ and tyrosine hydroxylase‑expressing cells

  • Authors:
    • Yinxiu Ding
    • Zixin Zhang
    • Jiangbo Ma
    • Hechun Xia
    • Yin Wang
    • Yinming Liu
    • Quanrui Ma
    • Tao Sun
    • Juan Liu

  • View Affiliations

  • Published online on: July 8, 2016     https://doi.org/10.3892/mmr.2016.5489
  • Pages: 1993-1999

  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Parkinson's disease (PD) is a severe neurodegenerative disorder. Although the detailed underlying molecular mechanism remains to be elucidated, the major pathological feature of PD is the loss of dopaminergic (DA) neurons of the substantia nigra. The use of donor stem cells to replace DA neurons may be a key breakthrough in the treatment of PD. In the present study, the growth kinetics of hippocampal neural stem cells (Hip‑NSCs) isolated from postnatal mice and cultured in vitro were observed, specifically the generation of cells expressing DA neuronal markers nuclear receptor related‑1 protein (Nurr1) and tyrosine hydroxylase (TH). It was revealed that Hip‑NSCs differentiated primarily into astrocytes when cultured in serum‑containing medium. However, in low serum conditions, the number of βIII tubulin‑positive neurons increased markedly. The proportion of Nurr1‑positive cells and TH‑positive neurons, significantly increased with increasing duration of directed differentiation of Hip‑NSCs (P=0.0187 and 0.0254, respectively). The results of the present study reveal that Hip‑NSCs may be induced to differentiate in vitro into neurons expressing Nurr1 and TH, known to be critical regulators of DA neuronal fate. Additionally, their expression may be necessary to facilitate neuronal maturation in vitro. These data suggest that Hip‑NSCs may serve as a source of DA neurons for cell therapy in patients diagnosed with PD.
View Figures
View References

Related Articles

Journal Cover

September-2016
Volume 14 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 7.6
  • CiteScore Rank: Q1: #82/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.647
  • SCImago Journal Rank (SJR): 0.781
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Ding Y, Zhang Z, Ma J, Xia H, Wang Y, Liu Y, Ma Q, Sun T and Liu J: Directed differentiation of postnatal hippocampal neural stem cells generates nuclear receptor related‑1 protein‑ and tyrosine hydroxylase‑expressing cells. Mol Med Rep 14: 1993-1999, 2016.
APA
Ding, Y., Zhang, Z., Ma, J., Xia, H., Wang, Y., Liu, Y. ... Liu, J. (2016). Directed differentiation of postnatal hippocampal neural stem cells generates nuclear receptor related‑1 protein‑ and tyrosine hydroxylase‑expressing cells. Molecular Medicine Reports, 14, 1993-1999. https://doi.org/10.3892/mmr.2016.5489
MLA
Ding, Y., Zhang, Z., Ma, J., Xia, H., Wang, Y., Liu, Y., Ma, Q., Sun, T., Liu, J."Directed differentiation of postnatal hippocampal neural stem cells generates nuclear receptor related‑1 protein‑ and tyrosine hydroxylase‑expressing cells". Molecular Medicine Reports 14.3 (2016): 1993-1999.
Chicago
Ding, Y., Zhang, Z., Ma, J., Xia, H., Wang, Y., Liu, Y., Ma, Q., Sun, T., Liu, J."Directed differentiation of postnatal hippocampal neural stem cells generates nuclear receptor related‑1 protein‑ and tyrosine hydroxylase‑expressing cells". Molecular Medicine Reports 14, no. 3 (2016): 1993-1999. https://doi.org/10.3892/mmr.2016.5489