Identifying the role of Wilms tumor 1 associated protein in cancer prediction using integrative genomic analyses

  • Authors:
    • Li‑Sheng Wu
    • Jia‑Yi Qian
    • Minghai Wang
    • Haiwei Yang
  • View Affiliations

  • Published online on: July 18, 2016     https://doi.org/10.3892/mmr.2016.5528
  • Pages: 2823-2831
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Abstract

The Wilms tumor suppressor, WT1 was first identified due to its essential role in the normal development of the human genitourinary system. Wilms tumor 1 associated protein (WTAP) was subsequently revealed to interact with WT1 using yeast two-hybrid screening. The present study identified 44 complete WTAP genes in the genomes of vertebrates, including fish, amphibians, birds and mammals. The vertebrate WTAP proteins clustered into the primate, rodent and teleost lineages using phylogenetic tree analysis. From 1,347 available SNPs in the human WTAP gene, 19 were identified to cause missense mutations. WTAP was expressed in bladder, blood, brain, breast, colorectal, esophagus, eye, head and neck, lung, ovarian, prostate, skin and soft tissue cancers. A total of 17 out of 328 microarrays demonstrated an association between WTAP gene expression and cancer prognosis. However, the association between WTAP gene expression and prognosis varied in distinct types of cancer, and even in identical types of cancer from separate microarray databases. By searching the Catalogue of Somatic Mutations in Cancer database, 65 somatic mutations were identified in the human WTAP gene from the cancer tissue samples. These results suggest that the function of WTAP in tumor formation may be multidimensional. Furthermore, signal transducer and activator of transcription 1, forkhead box protein O1, interferon regulatory factor 1, glucocorticoid receptor and peroxisome proliferator-activated receptor γ transcription factor binding sites were identified in the upstream (promoter) region of the human WTAP gene, suggesting that these transcription factors may be involved in WTAP functions in tumor formation.
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September-2016
Volume 14 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wu LS, Qian JY, Wang M and Yang H: Identifying the role of Wilms tumor 1 associated protein in cancer prediction using integrative genomic analyses. Mol Med Rep 14: 2823-2831, 2016.
APA
Wu, L., Qian, J., Wang, M., & Yang, H. (2016). Identifying the role of Wilms tumor 1 associated protein in cancer prediction using integrative genomic analyses. Molecular Medicine Reports, 14, 2823-2831. https://doi.org/10.3892/mmr.2016.5528
MLA
Wu, L., Qian, J., Wang, M., Yang, H."Identifying the role of Wilms tumor 1 associated protein in cancer prediction using integrative genomic analyses". Molecular Medicine Reports 14.3 (2016): 2823-2831.
Chicago
Wu, L., Qian, J., Wang, M., Yang, H."Identifying the role of Wilms tumor 1 associated protein in cancer prediction using integrative genomic analyses". Molecular Medicine Reports 14, no. 3 (2016): 2823-2831. https://doi.org/10.3892/mmr.2016.5528